Matches in Nanopublications for { ?s ?p "293T cells were harvested for a luciferase reporter assay for 24 hours after cotransfection with NFKB1-dependent luciferase reporter construct and RIPK3 pYCI construct. Ectopic expression of RIPK3 alone induced NFKB1 activation approximately sevenfold. The carboxy-terminal region of RIPK3 is essential for NFKB1 activation, as RIPK3 (1-251) and RIPK3 (1-436) were not able to activate NFKB1. RIPK3 (K50D), RIPK3 (82-518) and RIPK3 (287-518) induced higher luciferase activity than full length RIPK3." ?g. }
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- _5 value "293T cells were harvested for a luciferase reporter assay for 24 hours after cotransfection with NFKB1-dependent luciferase reporter construct and RIPK3 pYCI construct. Ectopic expression of RIPK3 alone induced NFKB1 activation approximately sevenfold. The carboxy-terminal region of RIPK3 is essential for NFKB1 activation, as RIPK3 (1-251) and RIPK3 (1-436) were not able to activate NFKB1. RIPK3 (K50D), RIPK3 (82-518) and RIPK3 (287-518) induced higher luciferase activity than full length RIPK3." provenance.
- _5 value "293T cells were harvested for a luciferase reporter assay for 24 hours after cotransfection with NFKB1-dependent luciferase reporter construct and RIPK3 pYCI construct. Ectopic expression of RIPK3 alone induced NFKB1 activation approximately sevenfold. The carboxy-terminal region of RIPK3 is essential for NFKB1 activation, as RIPK3 (1-251) and RIPK3 (1-436) were not able to activate NFKB1. RIPK3 (K50D), RIPK3 (82-518) and RIPK3 (287-518) induced higher luciferase activity than full length RIPK3." provenance.