Matches in Nanopublications for { ?s ?p "Blnk and Ifit1 showed no increase in either the HOXD13- or HOXA9-expressing cells. Given that Blnk had increased expression after transient HOXD13 expression, it is likely that the lack of a response may be due to low HOXD13 levels in this population of HOXD13 stable-expressing cells. Since the SQ RT-PCR assay is not as useful for detecting changes in expression of genes that are initially highly expressed in the EGFP-only cells, we did not test the expression of Fstl and Igfbp4. Ngef and Casp8ap2 had lower expression levels in the HOXA13/EGFP-expressing cells (Ngef, Fig. 2; Casp8ap2 decreased 1.3-fold, not shown). HOXD13- and HOXA9-expressing cells also had reduced expression of Ngef and Casp8ap2 (Fig. 7B) by a magnitude consistent with that observed by microarray for the HOXA13/EGFP-expressing cells (Table 3). Thus, these results demonstrate that group 13 HOX proteins were capable of upregulating a subset of the genes that HOXA9 could not, implying a paralog-specific regulatory effect. However, the repression of Ngef and Casp8ap2 was a shared property. " ?g. }
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- _4 value "Blnk and Ifit1 showed no increase in either the HOXD13- or HOXA9-expressing cells. Given that Blnk had increased expression after transient HOXD13 expression, it is likely that the lack of a response may be due to low HOXD13 levels in this population of HOXD13 stable-expressing cells. Since the SQ RT-PCR assay is not as useful for detecting changes in expression of genes that are initially highly expressed in the EGFP-only cells, we did not test the expression of Fstl and Igfbp4. Ngef and Casp8ap2 had lower expression levels in the HOXA13/EGFP-expressing cells (Ngef, Fig. 2; Casp8ap2 decreased 1.3-fold, not shown). HOXD13- and HOXA9-expressing cells also had reduced expression of Ngef and Casp8ap2 (Fig. 7B) by a magnitude consistent with that observed by microarray for the HOXA13/EGFP-expressing cells (Table 3). Thus, these results demonstrate that group 13 HOX proteins were capable of upregulating a subset of the genes that HOXA9 could not, implying a paralog-specific regulatory effect. However, the repression of Ngef and Casp8ap2 was a shared property. " provenance.
- _4 value "Blnk and Ifit1 showed no increase in either the HOXD13- or HOXA9-expressing cells. Given that Blnk had increased expression after transient HOXD13 expression, it is likely that the lack of a response may be due to low HOXD13 levels in this population of HOXD13 stable-expressing cells. Since the SQ RT-PCR assay is not as useful for detecting changes in expression of genes that are initially highly expressed in the EGFP-only cells, we did not test the expression of Fstl and Igfbp4. Ngef and Casp8ap2 had lower expression levels in the HOXA13/EGFP-expressing cells (Ngef, Fig. 2; Casp8ap2 decreased 1.3-fold, not shown). HOXD13- and HOXA9-expressing cells also had reduced expression of Ngef and Casp8ap2 (Fig. 7B) by a magnitude consistent with that observed by microarray for the HOXA13/EGFP-expressing cells (Table 3). Thus, these results demonstrate that group 13 HOX proteins were capable of upregulating a subset of the genes that HOXA9 could not, implying a paralog-specific regulatory effect. However, the repression of Ngef and Casp8ap2 was a shared property. " provenance.