Nanopublications LDF server

Matches in Nanopublications for { ?s ?p "HRG induces the phosphorylation of BRCA1, peaking at 0.5 to 2 hours and returning to basal level thereafter. In order to determine whether Cdk4 phosphorylates BRCA1, wild type Cdk4 cDNA and the kinase dead mutant Cdk4-D158N were transiently overexpressed in T47D cells. After 48 hours, total cell lysates were prepared and subsequently immunoprecipitated with anti-Cdk4 antibodies following 2 hours of HRG stimulation. The immunoprecipitates were incubated with wild type BRCA1 or with c-terminal truncated BRCA1. Only the wild type Cdk4 precipitated from HRG treated cells was able to phosphorylate BRCA1, while no phosphorylation was observed in untreated cells, in cells expressing kinase dead mutant or in cells expressing C-terminal truncated BRCA1." ?g. }

• _5 value "HRG induces the phosphorylation of BRCA1, peaking at 0.5 to 2 hours and returning to basal level thereafter. In order to determine whether Cdk4 phosphorylates BRCA1, wild type Cdk4 cDNA and the kinase dead mutant Cdk4-D158N were transiently overexpressed in T47D cells. After 48 hours, total cell lysates were prepared and subsequently immunoprecipitated with anti-Cdk4 antibodies following 2 hours of HRG stimulation. The immunoprecipitates were incubated with wild type BRCA1 or with c-terminal truncated BRCA1. Only the wild type Cdk4 precipitated from HRG treated cells was able to phosphorylate BRCA1, while no phosphorylation was observed in untreated cells, in cells expressing kinase dead mutant or in cells expressing C-terminal truncated BRCA1." provenance.
• _5 value "HRG induces the phosphorylation of BRCA1, peaking at 0.5 to 2 hours and returning to basal level thereafter. In order to determine whether Cdk4 phosphorylates BRCA1, wild type Cdk4 cDNA and the kinase dead mutant Cdk4-D158N were transiently overexpressed in T47D cells. After 48 hours, total cell lysates were prepared and subsequently immunoprecipitated with anti-Cdk4 antibodies following 2 hours of HRG stimulation. The immunoprecipitates were incubated with wild type BRCA1 or with c-terminal truncated BRCA1. Only the wild type Cdk4 precipitated from HRG treated cells was able to phosphorylate BRCA1, while no phosphorylation was observed in untreated cells, in cells expressing kinase dead mutant or in cells expressing C-terminal truncated BRCA1." provenance.