Matches in Nanopublications for { ?s ?p "PIAS1 inhibits TNF-{alpha}-induced NF-{kappa}B-dependent gene activation. (A) Luciferase (Luc.) reporter assays. Human 293T cells (left) or lung cancer A549 cells (right) were transiently transfected with luciferase reporter construct 2xNF-{kappa}B together with increasing amounts of Flag-PIAS1. Twenty-four hours posttransfection, cells were either untreated (gray bar) or treated with TNF-{alpha} for 6 h (black bar), and cell lysates were subjected to luciferase assays. Shown is the average of results from three independent experiments. Pias1 decreases NFkappaB activity in a dose dependent manner. " ?g. }
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- _4 value "PIAS1 inhibits TNF-{alpha}-induced NF-{kappa}B-dependent gene activation. (A) Luciferase (Luc.) reporter assays. Human 293T cells (left) or lung cancer A549 cells (right) were transiently transfected with luciferase reporter construct 2xNF-{kappa}B together with increasing amounts of Flag-PIAS1. Twenty-four hours posttransfection, cells were either untreated (gray bar) or treated with TNF-{alpha} for 6 h (black bar), and cell lysates were subjected to luciferase assays. Shown is the average of results from three independent experiments. Pias1 decreases NFkappaB activity in a dose dependent manner. " provenance.
- _4 value "PIAS1 inhibits TNF-{alpha}-induced NF-{kappa}B-dependent gene activation. (A) Luciferase (Luc.) reporter assays. Human 293T cells (left) or lung cancer A549 cells (right) were transiently transfected with luciferase reporter construct 2xNF-{kappa}B together with increasing amounts of Flag-PIAS1. Twenty-four hours posttransfection, cells were either untreated (gray bar) or treated with TNF-{alpha} for 6 h (black bar), and cell lysates were subjected to luciferase assays. Shown is the average of results from three independent experiments. Pias1 decreases NFkappaB activity in a dose dependent manner. " provenance.