Matches in Nanopublications for { ?s ?p "We have previously shown that in CTCL cells transfected with a dominant negative Stat3, the absence of endogenous SOCS-3 coincides with increased IFNalpha signaling.19 To provide direct evidence that SOCS-3 does indeed modulate the response to this cytokine, we examined IFNalpha responses in (i) wt CTCL, (ii) CTCL cells expressing inducible SOCS-3, and (iii) CTCL cells expressing the dominant negative Stat3 (Stat3D), which inhibits the endogenous SOCS-3 expression.19 In agreement with the difference seen previously between SOCS-3hi and SOCS-3lo cells,19 expression of SOCS-3 caused a marked reduction in IFNalpha-induced phosphorylation of both Stat1 and Stat3 (Figure 4a lanes 6 and 8, respectively), whereas induction of the nonactive SOCS-3 mutant had no effect (lanes 10 and 12). Figure 4 legend: SOCS-3 expression inhibits Stat activation induced by IFNalpha and IL-2, but not IL-4. " ?g. }
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- _7 value "We have previously shown that in CTCL cells transfected with a dominant negative Stat3, the absence of endogenous SOCS-3 coincides with increased IFNalpha signaling.19 To provide direct evidence that SOCS-3 does indeed modulate the response to this cytokine, we examined IFNalpha responses in (i) wt CTCL, (ii) CTCL cells expressing inducible SOCS-3, and (iii) CTCL cells expressing the dominant negative Stat3 (Stat3D), which inhibits the endogenous SOCS-3 expression.19 In agreement with the difference seen previously between SOCS-3hi and SOCS-3lo cells,19 expression of SOCS-3 caused a marked reduction in IFNalpha-induced phosphorylation of both Stat1 and Stat3 (Figure 4a lanes 6 and 8, respectively), whereas induction of the nonactive SOCS-3 mutant had no effect (lanes 10 and 12). Figure 4 legend: SOCS-3 expression inhibits Stat activation induced by IFNalpha and IL-2, but not IL-4. " provenance.
- _7 value "We have previously shown that in CTCL cells transfected with a dominant negative Stat3, the absence of endogenous SOCS-3 coincides with increased IFNalpha signaling.19 To provide direct evidence that SOCS-3 does indeed modulate the response to this cytokine, we examined IFNalpha responses in (i) wt CTCL, (ii) CTCL cells expressing inducible SOCS-3, and (iii) CTCL cells expressing the dominant negative Stat3 (Stat3D), which inhibits the endogenous SOCS-3 expression.19 In agreement with the difference seen previously between SOCS-3hi and SOCS-3lo cells,19 expression of SOCS-3 caused a marked reduction in IFNalpha-induced phosphorylation of both Stat1 and Stat3 (Figure 4a lanes 6 and 8, respectively), whereas induction of the nonactive SOCS-3 mutant had no effect (lanes 10 and 12). Figure 4 legend: SOCS-3 expression inhibits Stat activation induced by IFNalpha and IL-2, but not IL-4. " provenance.