Matches in Nanopublications for { ?s <http://www.w3.org/ns/prov#value> ?o ?g. }
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _4 value "The L-type Ca21 channel is activated by the b-adrenoceptor in the cells isolated from tracheal (Welling et al., 1992a, b), rabbit ear artery (Benham and Tsien, 1988), guinea pig taenia coli (Muraki et al., 1993), rat aorta (Neveu et al., 1994) and rabbit portal vein (Xiong et al., 1994). Although opening of the L-type Ca21 channels increase [Ca21]i, at least in a part of the smooth muscle cell, stimulation of the b-adrenoceptors induce relaxation but not contraction. This discrepancy may be explained by the increase in cyclic AMP and also by the presence of a noncontractile Ca21 compartment in the cell (see sections III.B. and IV.A.2.)." provenance.
- _4 value "In vivo, HNF4 DNA-binding activity is reduced by fasting and by inducers of intracellular cyclic AMP (cAMP) accumulation." provenance.
- _5 value "phosphorylation by PKA strongly repressed the binding affinity of the wild-type factor but not that of HNF4 mutants. Accordingly, in transfection assays, expression vectors for the mutated HNF4 proteins activated transcription more efficiently than that for the wild-type protein-when cotransfected with the PKA catalytic subunit expression vector. Therefore, HNF4 is a direct target of PKA which might be involved in the transcriptional inhibition of liver genes by cAMP inducers.from full text (mutated HNF4 proteins at positions 133 and 134 Serine." provenance.
- _5 value "phosphorylation by PKA strongly repressed the binding affinity of the wild-type factor but not that of HNF4 mutants. Accordingly, in transfection assays, expression vectors for the mutated HNF4 proteins activated transcription more efficiently than that for the wild-type protein-when cotransfected with the PKA catalytic subunit expression vector. Therefore, HNF4 is a direct target of PKA which might be involved in the transcriptional inhibition of liver genes by cAMP inducers.from full text (mutated HNF4 proteins at positions 133 and 134 Serine." provenance.
- _5 value "From mutations file" provenance.
- _4 value "Although IL-1a and TNFa caused induction of NF-kB DNA binding activity in both cell types,(By EMSA)" provenance.
- _5 value "Although IL-1a and TNFa caused induction of NF-kB DNA binding activity in both cell types,(By EMSA)" provenance.
- _6 value "However, the intensity of complex III (NFkB/DNA complex) was at least 10?15-fold (as judged by densitometric scanning) greater with nuclear extracts of A549/IkBR cells compared with that observed with extracts of control cells (Fig. 3, lower panel).....Because different members of the NF-kB-Rel family could potentially interact with the kB motifs, we used specific antibodies to determine the composition of the different polypeptide complexes. Formation of complex I was affected with both anti-p50 and anti-p65 antibodies, suggesting that it contained the classic p50-p65 heterodimer (lanes 8 and 9)." provenance.
- _6 value "Taken together, it appears that complex I is a heterodimer of p50-p65 and complex II contains p50 homodimers.....Collectively, the EMSAs showed that overexpression of IkBR decreases formation of complex II..." provenance.
- _5 value "CHUK associates with the NF-kappaB inhibitory protein, IkappaB-alpha, in mammalian cells. CHUK specifically phosphorylates IkappaB-alpha on both serine 32 and serine 36, modifications that are required for targeted degradation of IkappaB-alpha via the ubiquitin-proteasome pathway." provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _4 value "in vitro; in vivo; Two-hybrid" provenance.
- _4 value "Treatment of NSCLC cells with TGF-beta 1 and PMA resulted in a persistent increase in the expression of fibronectin mRNA and protein" provenance.
- _3 value "Prednisolone inhibited the production of IL-1 alpha, IL-1 beta, IL-6 and TNF alpha." provenance.
- _6 value "In contrast, SM-11044, a nonselective beta-adrenoreceptor agonist, stimulated colon relaxation more efficiently than [rat adipocyte] lipolysis" provenance.
- _3 value "Induction of C3 mRNA and protein by dbcAMP at 24 hours was inhibited 85% by a neutralizing anti-IL-1beta antibody and 76% with an IL-1 receptor antagonist. This suggests that dbcAMP-induced synthesis of IL-1beta mediates the C3 production by SchC in an autocrine/paracrine fashion by binding to a functional IL-1 receptor expressed on the surface of SchC." provenance.
- _3 value "We report here that interleukin-6 (IL-6), the principal cytokine mediating the synthesis of most acute-phase proteins in the liver, downregulates the production of factor XII by the human hepatoma cell line HepG2 by up to 75%. The decrease in protein secretion correlated with an equivalent decrease of factor XII mRNA likely indicating a pretranslational control of factor XII gene expression by IL-6." provenance.
- _5 value "Cyclindependent kinases (CDKs), mainly CDK1 associated with cyclin B, have been identified as the kinase system that phosphorylates Ser-25 and Ser-38, but the protein kinase involved in phosphorylation of Ser-16 and Ser-63 during mitosis remains to be identified (5, 21). Besides cell cycle-regulated phosphorylation, Op18 is phosphorylated by members of the mitogen-activated protein kinase family on Ser-25 (22-24), by the Ca21/calmodulin-dependent kinase IV/Gr (CaMK IV/Gr) on Ser-16 (25), and by the cyclic AMP-dependent protein kinase (PKA) on Ser-16 and Ser-63 (4)." provenance.
- _5 value "Here we report that glycogen synthase kinase 3 (GSK-3) is capable of phosphorylating IRS-1 and that this modification converts IRS-1 into an inhibitor of IR tyrosine kinase activity in vitro." provenance.
- _4 value "we evaluated insulin secretion in transgenic (Tg) mice overexpressing the liver-type subunit of phosphofructokinase (PFKL). Three independently derived Tg-PFKL lines showed random and postprandial hyperglycemia with diminished acute insulin response following intravenous glucose tolerance load" provenance.
- _3 value "Similar to that of KGF, expression of GPx was strongly induced after cutaneous injury" provenance.
- _3 value "Similar to that of KGF, expression of GPx was strongly induced after cutaneous injury" provenance.
- _3 value "Treatment with 17b-estradiol raised galectin-1 mRNA level as early as 6 hr and the high level of galectin-1 mRNA remained up to 24 hr (Fig. 2A)." provenance.
- _5 value "But after 24 hours, 185-4 cells expressed CD95 (Fas/Apo-1) on their surfaces on stimulation via Fc gammaRIII" provenance.
- _6 value "Protein-protein interaction studies reveal that this domain of p45 NF-E2 binds specifically to a component of the transcription initiation complex, TATA binding protein associated factor TAFII130." provenance.
- _5 value "E12N was more sensitive to calmodulin and S-100aa" provenance.
- _6 value "Modified assertion" provenance.
- _4 value "Miz-1 has a potent growth arrest function." provenance.
- _5 value "Modified assertion" provenance.
- _3 value "cAMP and Ca2+ acted together with the acute phase cytokine interleukin-1beta (IL_1beta) to inhibit hepatocyte DNA replication." provenance.
- _6 value "RHA was found to cooperate with CBP in mediating target gene activation via the CAMP responsive factor CREB" provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _4 value "Modified assertion" provenance.
- _4 value "Modified assertion" provenance.
- _5 value "CaM inhibits phosphorylation of EWS, TLS/FUS, and PSF by PKC." provenance.
- _5 value "Stimulation of cell lines as well as normal progenitor cells with SCF rapidly increased tyrosine phosphorylation of the Src family member Lyn. SCF also induced increases in Lyn kinase activity in vitro." provenance.
- _7 value "Co-purification; in vitro; in vivo" provenance.
- _5 value "\"TbetaRI phosphorylation of Smad2 on Ser465 and Ser467 is required for Smad2-Smad4 complex formation and signaling.\"" provenance.
- _5 value "\"TbetaRI phosphorylation of Smad2 on Ser465 and Ser467 is required for Smad2-Smad4 complex formation and signaling.\"" provenance.
- _7 value "Modified assertion" provenance.
- _4 value "Following actinomycin D treatment, the competition profiles of cytoplasmic and nuclear 35-kDa AUBP activity/hnRNP A1 were differentially modulated: cytoplasmic 35-kDa binding to radiolabeled GM-CSF RNA was less well competed by unlabeled eta-globin and DUP33Y5 RNA, while competition by GM-CSF was relatively unaffected (Fig. 5 A )." provenance.
- _6 value "Here, we have shown that MKK7, but not SEK1/ MKK4, is activated by Fas as an activator for JNK/ SAPK and that MKK6 is a major activator for p38 in Fas signaling" provenance.
- _5 value "Tyrosine phosphorylation of Eps8 was detected only at the permissive temperature." provenance.
- _5 value "Modified assertion" provenance.
- _4 value "Phosphorylation of p42 and p38 MAPK was not observed in cells treated with both EGF and TNF alpha." provenance.
- _3 value "Finally, when NIH 3T3 cells are grown in hypertonic medium, AR mRNA levels are significantly increased whereas FR-1 mRNA levels are only slightly up-regulated" provenance.
- _3 value "Our findings indicate that androgens stimulate the expression of SREBP transcripts and precursor proteins and enhance the nuclear content of the mature active form of the transcription factor" provenance.
- _3 value "SIL is expressed in proliferating cells and is down-regulated when cellular proliferation ceases because of serum starvation, contact inhibition, or induction of terminal differentiation." provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _5 value "BDNF binds to and activates the tyrosine kinase receptor, TrkB," provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _7 value "Similar results were obtained with protein kinase C, a serine/threonine kinase" provenance.
- _5 value "L-NMA inhibited poly(I-C)-induced surface expression of E-selectin and VCAM-1 by 25 and 45%, respectively, and mRNA levels of E-selectin and VCAM-1" provenance.
- _3 value "We found that expression of CDK2 and CDK4 genes were up-regulated within hours of androgen treatment as detected in Northern and Western blot analyses. Kinase assay also confirmed that there was increased CDK2 kinase activity upon androgen stimulation." provenance.
- _5 value "Activin A caused a dose-dependent inhibition of DNA synthesis and proliferation by LNCaP and androgen-independent DU145 cells" provenance.
- _5 value "growth inhibitory response to activins A and B by LNCaP and DU145 cells, and the ability of follistatin to block these effects" provenance.
- _6 value "HER2/Neu-positive breast tumors revealed that the HER2/Neu receptor tyrosine kinase initiated an intracellular signaling cascade resulting in increased PEA3 transcriptional activity; transcriptionally-activated PEA3 stimulated HER2/neu and PEA3 gene transcription by binding to sites in the promoters of these genes" provenance.
- _3 value "Quercetin down-regulated the expression of c-myc and Ki-ras oncogenes and led to induced differentiation and apoptosis in K562 cells." provenance.
- _4 value "Using altered specificity mutants, we directly compared the ability of Oct-1 and Oct-2 to regulate the activity of a histone H2B promoter in B cells." provenance.
- _5 value "Modified assertion" provenance.
- _4 value "Modified assertion" provenance.
- _3 value "Transgenic mice overexpressing a constitutively active human TGF-beta1 under control of the rat phosphoenolpyruvate carboxykinase regulatory sequences developed fibrosis of the liver, kidney, and adipose tissue, and exhibited a severe reduction in body fat." provenance.
- _5 value "PDGF-BB downregulates PKC-d and less pronounced also PKC- a after 30 min of incubation" provenance.
- _2 value "The increase in proliferative activity of renal cortical tissue from FA-injected rats, evaluated by immunostaining with Mib-1 antibody, was limited to tubular epithelial cells." provenance.
- _4 value "Moreover, BDNF or NT-4/5 treatment stimulated the expression of GAP-43 and T alpha1-tubulin mRNA and maintained the level of trkB expression." provenance.
- _4 value "Moreover, BDNF or NT-4/5 treatment stimulated the expression of GAP-43 and T alpha1-tubulin mRNA and maintained the level of trkB expression." provenance.
- _7 value "Integrin alpha1-blocking and alpha2-blocking antibodies (Ab) each partially inhibited attachment of microvascular EC to collagen I, and alpha1-blocking Ab also inhibited attachment to collagen IV and laminin-1. Induction of alpha1beta1 and alpha2beta1 expression by VEGF promoted cell spreading on collagen I gels which was abolished by a combination of alpha1-blocking and alpha2-blocking Abs. In vivo, a combination of alpha1-blocking and alpha2-blocking Abs markedly inhibited VEGF-driven angiogenesis;" provenance.
- _4 value "Expression of SFRP1 decreases the intracellular concentration of CTNNB1. Immunohistochemistry analysis reveals a lower concentration of CTNNB1 in the SFRP1 transfectants." provenance.
- _6 value "JNK1 associates with p53 in vivo, with or without activation, confirming that JNK1 is indeed a p53 kinase. Interestingly, activated JNK2 and JNK3 also phosphorylate serine 34 of mouse p53. Furthermore, JNK2 and JNK3 also associate with p53 in vivo, indicating that not only JNK1, but also JNK2 and JNK3 are p53 N-terminal serine 34 kinases" provenance.
- _5 value "In vivo 32P labeling and mutagenesis demonstrated that m/p-PKBalpha activity was due to phosphorylation on Thr308 and Ser473, that are normally induced on PKB following stimulation of the cells with insulin or insulin-like growth factor-1 (IGF-1).... Following activation the kinase detached from the membrane and translocated to the nucleus." provenance.
- _6 value "In vivo 32P labeling and mutagenesis demonstrated that m/p-PKBalpha activity was due to phosphorylation on Thr308 and Ser473, that are normally induced on PKB following stimulation of the cells with insulin or insulin-like growth factor-1 (IGF-1).... Following activation the kinase detached from the membrane and translocated to the nucleus." provenance.
- _2 value "Hypoxia induces vasoconstriction, in part, by down-regulating endothelial cell nitric oxide synthase (ecNOS) expression." provenance.
- _6 value "Although Enslen et al. ( 86 ) recently reported that the CaMKK/CaMK IV cascade can also activate the MAP kinases JNK1, ERK2, and p38/Mpk2 and subsequently stimulate transcription dependent on phosphorylation of c- jun , Elk-1, and ATF-2, we conclude that the CaMK IV activity induced in T lymphocytes through stimulation of the CD5 receptor directly up-regulates the c- fos expression through phosphorylation of either CREB or SRF and is independent of the activation of MAP kinases." provenance.
- _5 value "Overexpression of EGR-4 mediates repression of a minimal c-fos promoter through a threefold EGR consensus site. Furthermore the EGR-4 protein displays autoregulatory activities. This protein downregulates expression of its own gene promoter in a dose dependent manner." provenance.
- _5 value "Overexpression of EGR-4 mediates repression of a minimal c-fos promoter through a threefold EGR consensus site. Furthermore the EGR-4 protein displays autoregulatory activities. This protein downregulates expression of its own gene promoter in a dose dependent manner." provenance.
- _3 value "type 2, type 3 and type 1 desmosomal cadherin messages are not obligatorily expressed as pairs during stratified epithelial morphogenesis. Instead the individual genes appear to be transcribed in hierarchical, overlapping temporal and spatial patterns extending from DSG2 to DSC1" provenance.
- _3 value "type 2, type 3 and type 1 desmosomal cadherin messages are not obligatorily expressed as pairs during stratified epithelial morphogenesis. Instead the individual genes appear to be transcribed in hierarchical, overlapping temporal and spatial patterns extending from DSG2 to DSC1" provenance.
- _3 value "type 2, type 3 and type 1 desmosomal cadherin messages are not obligatorily expressed as pairs during stratified epithelial morphogenesis. Instead the individual genes appear to be transcribed in hierarchical, overlapping temporal and spatial patterns extending from DSG2 to DSC1" provenance.
- _7 value "The TNF-induced death of mouse primary fibroblasts expressing deregulated c-Myc was inhibited by transient overexpression of the p65 subunit of NF-kappaB, which increased NF-kappaB activity in the cells." provenance.
- _5 value "Modified assertion" provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _5 value "IL-6 induced the activation and tyrosine phosphorylation of p59Fyn, p56/59Hck, and p56Lyn." provenance.
- _5 value "In particular, the protein kinase A-induced phosphorylation of nuclear proteins, such as CREB or CREM, could be responsible for transcriptional effects via CREs present in gene promoters" provenance.
- _5 value "The binding of CTCF to the APBbeta domain was further established by competition with CTCF binding oligonucleotides in mobility shift electrophoresis. The identity was also confirmed by the observation that the APBbeta binding factor is recognized by antibodies against C- and N-terminal sequences of CTCF. In addition, oligonucleotide competition during in vitro transcription affirmed that CTCF acts as a transcriptional activator in the APP gene promoter." provenance.
- _4 value "The involvement of AP1 as well as Ets transcription factors was confirmed by examining different promoter constructs containing deletions in either the AP-1 or the PEA3 element, and by using an expression plasmid for dominant negative Ets-2." provenance.
- _6 value "The addition of recombinant negative cofactor NC2 (NC2alpha/DRAP1 and NC2beta/Dr1) to the reconstituted transcription system restored the activity of the AREB6 repression domain. We further demonstrated interactions between the AREB6 repression domain and NC2alpha in yeast two-hybrid assay" provenance.
- _3 value "Estradiol stimulates expression of mouse endometrial Muc1, an effect that is inhibited by administration of progesterone." provenance.
- _6 value "we demonstrate that binding of both lipoprotein and non-lipoprotein ligands to MSR-A induced protein tyrosine phosphorylation and increased protein kinase C (PKC) activity leading to up-regulated urokinase-type plasminogen activator (uPA) expression" provenance.
- _6 value "Here, we report that the catalytic subunit of cAMP-dependent protein kinase (PKA) but not casein kinase II or protein kinase C phosphorylates recombinant human acetylcholinesterase (AChE) in vitro. This enhances acetylthiocholine hydrolysis up to 10-fold as compared to untreated AChE, while leaving unaffected the enzyme's affinity for this substrate and for various active and peripheral site inhibitors. Phosphorylation of AChE may possibly offer a rapid feedback mechanism that can compensate for impairments in cholinergic neurotransmission, modulating the hydrolytic activity of this enzyme and enabling acetylcholine hydrolysis to proceed under such challenges." provenance.
- _4 value "Serum-starved 3T3-F442A fibroblasts stimulated with growth hormone induces the expression of Socs-1, by comparision, was much less pronounced although with similar kinetics." provenance.
- _4 value "Serum-starved 3T3-F442A fibroblasts stimulated with growth hormone resulted in the upregulation of CIS mRNA." provenance.
- _3 value "Here we identify six sites in MAPKAP-K1a that become phosphorylated in transfected COS-1 cells. Stimulation with phorbol 12-myristate 13-acetate induces the phosphorylation of Thr360, Ser364, Thr574, and Ser381 and increases the phosphorylation of Ser222 and Ser733." provenance.
- _6 value "The p38 MAP kinase kinase MKK6 is identified as a common activator of p38 alpha, p38 beta 2, and p38 gamma MAP kinase isoforms, while MKK3 activates only p38 alpha and p38 gamma MAP kinase isoforms." provenance.
- _6 value "The p38 MAP kinase kinase MKK6 is identified as a common activator of p38 alpha, p38 beta 2, and p38 gamma MAP kinase isoforms, while MKK3 activates only p38 alpha and p38 gamma MAP kinase isoforms." provenance.
- _4 value "Finally, NGF was shown to upregulate tenascin-R expression in PC12 cells, indicating that expression of neuron-derived tenascin-R may be regulated by exogenous NGF." provenance.
- _5 value "These results suggest that PACAP binds to two binding sites, PACAP type I receptors and PACAP type II receptors." provenance.
- _5 value "Caveolin-1 and -3 were abundantly expressed in the heart and skeletal muscles, but not in the brain. Our data suggest that the expression of caveolin subtypes is regulated by beta-adrenergic receptor stimulation in the heart." provenance.