Matches in Nanopublications for { ?s <http://www.w3.org/ns/prov#value> ?o ?g. }
- _4 value "Ariadne: Thus, the major components of the AP-1 in MEK-DD-transformed CEFs were shown to be Fra-2 and c-Jun, and Fra-2 was highly phosphorylated, as reported in CEFs transformed by v- src , Ha- ras , and activated raf (17 , 22) . [Regulation] Here, we report that the transcriptional activity of Fra-2/c-Jun heterodimer was greatly enhanced by cotransfecting a constitutively active mutant of MEK1 gene (MEK-DD) into F9 cells, indicating that Fra-2 was converted into an active transactivator after phosphorylation by MAPK." provenance.
- _5 value "Ariadne: Thus, the major components of the AP-1 in MEK-DD-transformed CEFs were shown to be Fra-2 and c-Jun, and Fra-2 was highly phosphorylated, as reported in CEFs transformed by v- src , Ha- ras , and activated raf (17 , 22) . [Regulation] Here, we report that the transcriptional activity of Fra-2/c-Jun heterodimer was greatly enhanced by cotransfecting a constitutively active mutant of MEK1 gene (MEK-DD) into F9 cells, indicating that Fra-2 was converted into an active transactivator after phosphorylation by MAPK." provenance.
- _7 value "The TNFR-I/Grb2 interaction is essential for the TNF-alpha-dependent activation of c-Raf-1 kinase" provenance.
- _3 value "A basal expression of NOS II mRNA and protein was detected in skeletal muscle from untreated wild-type mice; expression increased when mice were treated with bacterial lipopolysaccharide (LPS)." provenance.
- _4 value "Here we report that murine macrophages stimulated to produce NO with lipopolysaccharide and interferon-gamma express the betaminor hemoglobin subunit. Consumption of NO, however, was not increased by cytokines or by hemoglobin expression. These data suggest alternative functions for globins in mammalian cells, and they challenge the prevailing view that the expression of alpha- and beta-globin genes is always balanced and coordinated." provenance.
- _4 value "Notch1 activates Hes1 promoter, a downstream target of the notch signaling pathway. Activation of Hes1 promoter was reduced by 70% in PS1-KO cells relative to PS1-wt cells, suggesting that PS1 potentiates Notch1 signaling activity." provenance.
- _5 value "Pituitary corticotroph SOCS-3 is inhibiting LIF-activated Janus kinase-signal transducers and activators of transcription (STAT) signaling in a negative autoregulatory loop. We now demonstrate in corticotroph AtT-20 cells that LIF-stimulated endogenous SOCS-3 mRNA expression is blocked in stable transfectants of SOCS-3 wild type or in dominant negative STAT-3 mutants, respectively." provenance.
- _3 value "Constitutively produced NO promoted tumor-cell invasiveness in vitro by down-regulating TIMP 2 and TIMP 3. In addition, there was up-regulation of MMP-2, when extra NO was induced by IFN-gamma and LPS" provenance.
- _5 value "However, K8/19 tyrosine phosphorylation is independent of EGF signaling or ERK activation while inhibition of p38 kinase activity blocks pervanadate-induced K8/19 tyrosine phosphorylation" provenance.
- _3 value "Acute stress or direct glucocorticoid administration raises muscle GS mRNA levels dramatically without a parallel increase in GS protein levels." provenance.
- _5 value "An XO enzyme activity of 5.0 mU/ml led to a twofold increase in IL-6 secretion in NHBE cells. And incubation of HS-24 cells with XO (20 mU/ml) induced a 1.8-fold increase in IL-6 production " provenance.
- _5 value "Measurement of IL-6 levels in the supernatants demonstrated that TNF-a (200 U/ml) caused a markedly enhanced production of IL-6, and increasing DMSO concentrations resulted in an essentially linear dose-dependent suppression of TNF-ainduced IL-6 production (Fig. 5, B and D). " provenance.
- _4 value "TNF-a was dose dependently capable of inducing IL-6 release from NHBE and HS-24 cells (Fig. 5, A and C). " provenance.
- _5 value "OsM activates Stat1, Stat3, and MAPK." provenance.
- _5 value "OsM activates Stat1, Stat3, and MAPK." provenance.
- _3 value "FGF treatment of RCS cells induces phosphorylation of STAT-1, its translocation to the nucleus, and an increase in the expression of the cell-cycle inhibitor p21WAF1/CIP1." provenance.
- _5 value "FGF treatment of RCS cells induces phosphorylation of STAT-1, its translocation to the nucleus, and an increase in the expression of the cell-cycle inhibitor p21WAF1/CIP1." provenance.
- _3 value "We show here that activation of FGF signaling inhibits chondrocyte proliferation both in a rat chondrosarcoma (RCS) cell line and in primary murine chondrocytes." provenance.
- _7 value "The rapamycin-sensitive component in the 4E-BP1 phosphorylation pathway is FRAP/mTOR (FKBP12-rapamycin associated protein/mammalian target of rapamycin), also known as RAFT1 (rapamycin and 12-kD FK506 binding protein target 1), a member of the PIK (phosphoinositide kinase-related) family of kinases. (from full text) A recombinant FRAP/mTOR protein and a FRAP/mTOR immunoprecipitate were utilized in in vitro kinase assays to phosphorylate 4E-BP1. Phosphopeptide mapping of the in vitro-labeled protein yielded two 4E-BP1 phosphopeptides that comigrated with phosphopeptides produced in vivo. Mass spectrometry analysis indicated that these peptides contain phosphorylated Thr-37 and Thr-46. Thr-37 and Thr-46 are efficiently phosphorylated in vitro by FRAP/mTOR when 4E-BP1 is bound to eIF4E." provenance.
- _5 value "The rapamycin-sensitive component in the 4E-BP1 phosphorylation pathway is FRAP/mTOR (FKBP12-rapamycin associated protein/mammalian target of rapamycin), also known as RAFT1 (rapamycin and 12-kD FK506 binding protein target 1), a member of the PIK (phosphoinositide kinase-related) family of kinases. (from full text) A recombinant FRAP/mTOR protein and a FRAP/mTOR immunoprecipitate were utilized in in vitro kinase assays to phosphorylate 4E-BP1. Phosphopeptide mapping of the in vitro-labeled protein yielded two 4E-BP1 phosphopeptides that comigrated with phosphopeptides produced in vivo. Mass spectrometry analysis indicated that these peptides contain phosphorylated Thr-37 and Thr-46. Thr-37 and Thr-46 are efficiently phosphorylated in vitro by FRAP/mTOR when 4E-BP1 is bound to eIF4E." provenance.
- _3 value "Insulin induces Egr-1 mRNA" provenance.
- _5 value "Furthermore, the phosphoinositide 3-kinase (PI3K) inhibitors wortmannin and LY294002 block Rac2 activation elicited by the receptor agonists, but not that by PMA. Thus the Gi-coupled receptors likely mediate Rac2 activation via PI3K, whereas PMA activates Rac2 in a PI3K-independent manner" provenance.
- _5 value "stimulation of human neutrophils with the Gi-coupled receptor agonists N-formyl-methionyl-leucyl-phenylalanine (fMLP) and leukotriene B4 (LTB4) leads to a rapid and transient increase in the GTP-bound state of Rac2, whereas phorbol myristate acetate (PMA) causes a slow but more sustained activation of Rac2" provenance.
- _3 value "Time course studies revealed the sustained induction of GCF2 after injury while PDGF-A levels sharply returned to baseline. Smooth muscle cell proliferation was inhibited by GCF2, an effect reversed by the addition of exogenous PDGF-AA." provenance.
- _4 value "IL-13 inhibited collagenase 3 production, IL-4 had little effect, and IL-10 had none." provenance.
- _4 value "Treatment of OA chondrocytes with a PKC agonist PMA (phorbol-12-myristate-13-acetate) induced the collagenase 3 production. TGFbeta2 reduced the PMA-induced collagenase 3 production in both L (low collagenase 3 basal synthesis and high IL-1beta inducibility) and H (high basal collagenase 3 levels and low IL-1beta inducibility) chondrocytes." provenance.
- _4 value "AP-1 activity, a crucial target involved in the triiodothyronine myogenic influence, repressed BTG1 expression, thus probably explaining the low BTG1 expression level in proliferating myoblasts" provenance.
- _3 value "the stimulation of BTG1 nuclear accumulation by T3 or 8-Br-cAMP probably results from an increased nuclear import or retention in the nucleus. Lastly, BTG1 overexpression in quail myoblasts mimicked the T3 or 8-Br-cAMP myogenic influence: (i) inhibition of myoblast proliferation due to an increased rate of myoblast withdrawal from the cell cycle; and (ii) stimulation of terminal differentiation" provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _6 value "Cells transfected with IRS-1 had increased levels of DNA synthesis, PCNA, GAPDH, and activated MAPK" provenance.
- _4 value "ApoA1 was significantly increased at 2 weeks of the growth hormone secretagogue MK-677 treatment in obese males." provenance.
- _2 value "ASP and insulin inhibited basal and norepinephrine-induced FFA release by stimulating fractional FFA re-esterification (both to the same extent) and by inhibiting FFA produced during lipolysis" provenance.
- _5 value "Selective PDE3 inhibition reversed the effects of both ASP and insulin on fractional FFA re-esterification and lipolysis" provenance.
- _5 value "MEKK1 also stimulates the transcriptional activity of the androgen receptor in the presence or absence of ligand, whereas a dominant negative mutant of MEKK1 impairs activation of the androgen receptor by androgen." provenance.
- _5 value "Here, we report that APS was tyrosine phosphorylated by Janus kinase-2 (JAK2) at its C-terminal tyrosine residue and interacted with c-Cbl." provenance.
- _4 value "NDF and Betacellulin (BTC) induced increase in tyrosine phosphorylation of ErbB3. NDF induced the strongest increase in the ErbB3 phosphotyrosine." provenance.
- _3 value "MT1-MMP mRNA levels increased within 6 h of exposure to oxidised LDL and remained elevated for 24 h. Treatment of cells with Ox-LDL increased (2 fold) the levels of MT1-MMP proteins in endothelial membranes. The levels of MT1-MMP protein were increased about 3.1 fold in endothelial cells treated with a combination of TNFalpha and ox-LDL." provenance.
- _3 value "These EC adhesion molecules include vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule 1 (ICAM-1), E-selectin, and P-selectin.6,7" provenance.
- _3 value "Treatment of female mice with testosterone increases the amounts of AO mRNA and of the relative translation product to levels similar to those in male animals." provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _4 value "caffeine, a non-specific phosphodiesterase (PDE) inhibitor" provenance.
- _3 value "We could demonstrate that alpha-tocopherol down-regulates scavenger receptor activity in macrophages in a dose-dependent manner. Scavenger receptor activity was reduced by 13, 16, 18 and 24% in the presence of 1, 5, 10 and 50 microM alpha-tocopherol, respectively. This effect was associated with a reduced SR-A mRNA expression and activity of AP-1 binding transcription factors in the presence of alpha-tocopherol" provenance.
- _3 value "Unlike other E1B19K binding BH3 proteins so far characterized, B5 does not induce apoptosis, but inhibits apoptosis induced by Nip3" provenance.
- _5 value "Modified assertion" provenance.
- _4 value "HER2-overexpressing cell lines and cells with coexpression of HER2 and HER3 receptors exhibited elevated levels of paxillin, as compared to low HER2 expressing cell lines or HER2 expression with undetectable HER3 level, paxillin expression dose not correlate well with HER1 expression." provenance.
- _4 value "This induction was consistent with an observed significant up-regulation of PDE4 activity." provenance.
- _6 value "In a later complementary study, Cheng et al. (1999) observed that assembly of cyclin D1/D2-CDK4 complexes was impaired in primary mouse embryo fibroblast (MEF) strains taken from animals lacking the p21 gene, the p27 gene, or both. " provenance.
- _6 value "NF-kappaB activation by tumor necrosis factor alpha (TNFalpha) provides a survival signal that impairs the TNFalpha-induced apoptotic response. We show here that expression of Par-4 inhibits the TNFalpha-induced nuclear translocation of p65 as well as the kappaB-dependent promoter activity." provenance.
- _4 value "NF-kappaB activation by tumor necrosis factor alpha (TNFalpha) provides a survival signal that impairs the TNFalpha-induced apoptotic response. We show here that expression of Par-4 inhibits the TNFalpha-induced nuclear translocation of p65 as well as the kappaB-dependent promoter activity." provenance.
- _6 value "Of potential functional relevance, the expression of Par-4 allows TNFalpha to induce apoptosis in NIH-3T3 cells. In addition, the down-regulation of Par-4 levels by oncogenic Ras sensitizes cells to TNFalpha-induced NF-kappaB activation." provenance.
- _6 value "JAK2 tyrosine kinase and the Src family p59 Fyn tyrosine kinase are required for Ang II-induced STAT1 tyrosine phosphorylation in VSMCs." provenance.
- _6 value "JAK2 tyrosine kinase and the Src family p59 Fyn tyrosine kinase are required for Ang II-induced STAT1 tyrosine phosphorylation in VSMCs." provenance.
- _6 value "FIGURE 4. Vav-mediated JNK1 activation is Rac, but not Ras, dependent. " provenance.
- _5 value "The complexity of IFN-mediated regulation of the murine Ly-6E gene in T cell lines is highlighted by the following observations: 1) multiple regulatory regions are present within different parts of the Ly-6E promoter and are necessary for IFN inducibility of the Ly-6E gene, 2) multiple transcription factors including Oct-1 and Oct-2 and the high mobility group (HMG) protein HMGI(Y) bind to regulatory elements present within the G region required for both IFN-alphabeta and IFN-gamma responses," provenance.
- _4 value "located in a CpG island. There are numerous binding sites for developmental and cell-type-specific transcription factors, including AP-1, AP-2, GATA, and several potential Oct 1 binding domains." provenance.
- _5 value "Analysis of eps8 phosphotyrosine content in v-Src transformed cells (IV5) revealed that both isoforms were highly tyrosyl phosphorylated and their readiness to be phosphorylated by Src in vitro further indicated that they were putative Src substrates as well. " provenance.
- _3 value "These antibodies revealed a protein with the expected 43 kDa molecular mass, up-regulated by phorbol ester, retinoids and 1,25-(OH)2 vitamin D3 in U937 cells." provenance.
- _5 value "These antibodies revealed a protein with the expected 43 kDa molecular mass, up-regulated by phorbol ester, retinoids and 1,25-(OH)2 vitamin D3 in U937 cells." provenance.
- _4 value "Caspase-3 also cleaves and activates gelsolin, a protein that regulates actin dynamics. Interestingly, activated gelsolin promotes both cytoplasmic and nuclear apoptosis, including DNA fragmentation." provenance.
- _4 value "Caspase-dependent activation of kinases including PAK2, MEKK1, and PKCd also promotes cytoplasmic and nuclear apoptosis. Interestingly, MEKK1 activation enhances caspase activation, suggesting that kinases may amplify or initiate the caspase cascade." provenance.
- _4 value "Caspase-dependent activation of kinases including PAK2, MEKK1, and PKCd also promotes cytoplasmic and nuclear apoptosis. Interestingly, MEKK1 activation enhances caspase activation, suggesting that kinases may amplify or initiate the caspase cascade." provenance.
- _5 value "Mutation of either of these putative NF-Y binding sites attenuates the transcriptional activation of SCD1 or SCD2 promoter-reporter genes in response to cellular sterol deprivation. Induction of both reporter genes is also attenuated when cells are cotransfected with dominant-negative forms of either NF-Y or SREBP. " provenance.
- _5 value "Mutation of either of these putative NF-Y binding sites attenuates the transcriptional activation of SCD1 or SCD2 promoter-reporter genes in response to cellular sterol deprivation. Induction of both reporter genes is also attenuated when cells are cotransfected with dominant-negative forms of either NF-Y or SREBP. " provenance.
- _4 value "Mutation of either of these putative NF-Y binding sites attenuates the transcriptional activation of SCD1 or SCD2 promoter-reporter genes in response to cellular sterol deprivation. Induction of both reporter genes is also attenuated when cells are cotransfected with dominant-negative forms of either NF-Y or SREBP. " provenance.
- _4 value "Mutation of either of these putative NF-Y binding sites attenuates the transcriptional activation of SCD1 or SCD2 promoter-reporter genes in response to cellular sterol deprivation. Induction of both reporter genes is also attenuated when cells are cotransfected with dominant-negative forms of either NF-Y or SREBP. " provenance.
- _3 value "In mouse fibroblasts adhered to substrates, PTP36 was phosphorylated on serine residues. PTP36 was found to make complexes with serine/threonine kinase(s), which phosphorylated PTP36 in vitro. PTP36 was dephosphorylated rapidly when the cell-substrate adhesion was disrupted and it was phosphorylated again along with the reattachment of the cells to fibronectin." provenance.
- _3 value "Staurosporine and ML-9, which inhibited the phosphorylation of PTP36 in vivo, induced the translocation of PTP36 too." provenance.
- _3 value "The relative contributions of superoxide anion (O2-) and peroxynitrite (PN) were evaluated in the pathogenesis of intestinal microvascular damage caused by the intravenous injection of E. coli lipopolysaccharide (LPS) in rats. The intravenous injection of LPS elicited an inflammatory response that was characterized by a time-dependent infiltration of neutrophils, lipid peroxidation, microvascular leakage (indicative of microvascular damage), and epithelial cell injury in both the duodenum and jejunum. Administration of the SODm SC-55858, FeTMPS or FeTMPyP at 3 h post LPS reduced the subsequent increase in microvascular leakage, lipid peroxidation and epithelial cell injury.% Our results suggest that O2 and peroxynitrite play a significant role in the pathogenesis of duodenal and intestinal injury during endotoxaemia and that their remoyal by SODm and peroxynitrite decomposition catalysts offers a novel approach to the treatment of septic shock or clinical conditions of gastrointestinal inflammation." provenance.
- _5 value "Modified assertion" provenance.
- _6 value "However, EGF-mediated membrane hyperpolarization is attenuated and Ca(2+) entry abolished in cells expressing annexin VI." provenance.
- _4 value "AR protein levels, AR activity, sorbitol production, and PKCalpha protein content were also greater in the MCGT1 cells" provenance.
- _4 value "Bcl-2 or Bcl-xL to function as inhibitors of apoptosis" provenance.
- _4 value "Expression of activated MEK completely blocked apoptosis induced by serum withdrawal (Fig. 5). In addition, the expression of active Ras and Raf-1, which also activate MEK, similarly inhibited cell death induced by growth factor deprivation" provenance.
- _4 value "Phosphorylation of glycogen synthase kinase-3 (GSK-3) by Akt has also been reported to contribute to cell survival (36)," provenance.
- _2 value "Similar results were obtained in both Rat-1 and Rat-1/B-Raf cells when apoptosis was induced by treatment with the PI 3-kinase inhibitor LY294002 in the presence of serum" provenance.
- _3 value "overexpressed B-Raf blocked cell death induced by PI 3-kinase inhibitors" provenance.
- _3 value "Insulin induced activation of ERK1/ERK2 was completely blocked by PD98059." provenance.
- _3 value "Insulin induced activation of ERK1/ERK2 was completely blocked by PD98059." provenance.
- _6 value "SHP-2 acts as a phosphatase on the JAK/STAT pathway and serves as linker to the MAP kinase pathway" provenance.
- _4 value "We show here that PGC-1, a cold-inducible coactivator of nuclear receptors, stimulates mitochondrial biogenesis and respiration in muscle cells through an induction of uncoupling protein 2 (UCP-2) and through regulation of the nuclear respiratory factors (NRFs). PGC-1 stimulates a powerful induction of NRF-1 and NRF-2 gene expression; in addition, PGC-1 binds to and coactivates the transcriptional function of NRF-1 on the promoter for mitochondrial transcription factor A (mtTFA)" provenance.
- _5 value "We show here that PGC-1, a cold-inducible coactivator of nuclear receptors, stimulates mitochondrial biogenesis and respiration in muscle cells through an induction of uncoupling protein 2 (UCP-2) and through regulation of the nuclear respiratory factors (NRFs). PGC-1 stimulates a powerful induction of NRF-1 and NRF-2 gene expression; in addition, PGC-1 binds to and coactivates the transcriptional function of NRF-1 on the promoter for mitochondrial transcription factor A (mtTFA)" provenance.
- _7 value "Stimulation of protein kinase C (PKC) in vivo decreased the binding of p97 to AldA-NRE and stimulated transcription, while inhibition of PKC stimulated p97 binding and downregulated transcription." provenance.
- _4 value "Only NGF slightly increased fractalkine message expression in hypothalamic neurons." provenance.
- _3 value "mechanical strain increases tenascin-C gene transcription by activating nuclear factor-kappaB through reactive oxygen species." provenance.
- _3 value "Table 1. Identification of Homocysteine-Respondent Genes Using cDNA Microarrays in Primary HUVECs Exposed to 5 mmol/L Homocysteine for 4 hours (18 hours timepoint is not curtaed)" provenance.
- _4 value "Specific antibodies against the subunits of alpha2beta1 integrins, the major collagen I receptor, induced partial inhibition of MMP2 activation. Treatment of cells with concanavalin A resulted in a marked activation of MMP2 that correlated with the proteolytic processing of MT1-MMP, the MMP2 activator, from a Mr=60 kd toward a Mr=43 kd polypeptide." provenance.
- _8 value "Specific antibodies against the subunits of alpha2beta1 integrins, the major collagen I receptor, induced partial inhibition of MMP2 activation. Treatment of cells with concanavalin A resulted in a marked activation of MMP2 that correlated with the proteolytic processing of MT1-MMP, the MMP2 activator, from a Mr=60 kd toward a Mr=43 kd polypeptide." provenance.
- _5 value "Overexpression of wild-type IKK-i phosphorylated serine residues Ser32 and Ser36 of IkappaB-alpha (preferentially Ser36), and significantly stimulated NF-kappaB activation. " provenance.
- _5 value "Overexpression of wild-type IKK-i phosphorylated serine residues Ser32 and Ser36 of IkappaB-alpha (preferentially Ser36), and significantly stimulated NF-kappaB activation. " provenance.
- _3 value "The cyclooxygenase inhibitor indomethacin inhibited prostaglandin E2 production, and further enhanced thapsigargin-induced tumor necrosis factor-alpha production in parallel with further increase in cell-associated PAF production." provenance.
- _3 value "Levels above normal were seen for alpha(2)-antiplasmin and factor X at 48 h, normalizing at 72 h" provenance.
- _3 value "alpha(1)-Macroglobulin (Pzp) and alpha(1)-protease inhibitor gradually decreased during the whole study period, probably being second line defense inhibitors." provenance.
- _3 value "Tumour cell metastatic potential is significantly enhanced following treatment with HGF/SF, the ligand for the c-met receptor tyrosine kinase." provenance.
- _7 value "Hypoxia inducible factor-1 (HIF-1) is a transcription factor composed of two subunits, HIF-1alpha and ARNT, which is activated under hypoxia." provenance.
- _4 value "We show that SRF activation by LIM kinase-1 is dependent on its ability to regulate actin treadmilling." provenance.
- _3 value "Here we show that Stat5 is essential for the high erythropoietic rate during fetal development. Stat5a-/-5b-/- embryos are severely anemic; erythroid progenitors are present in low numbers, show higher levels of apoptosis, and are less responsive to Epo. " provenance.
- _6 value "Using western immunobloting, we also observed the concurrent translocation of 5-LO protein from cytosol to membrane, an indication of its activation" provenance.
- _3 value "We treated rats for 10 days either with corticosterone (implanted subcutaneously) or with dexamethasone (injected daily); they were killed on day 10 after pellet implantation or 24 h after the 10th dexamethasone injection. We found increased levels of 5-LO mRNA and protein in hippocampus and cerebellum of glucocorticoid-treated rats" provenance.
- _2 value "Selective inhibition of PDE1C, but not of PDE4, augmented glucose-stimulated insulin secretion in a dose-dependent fashion thus demonstrating that PDE1C is the major PDE counteracting glucose-dependent insulin secretion from betaTC3 cells" provenance.
- _7 value "we also demonstrate that HMG-I/R is important for retinoid dependent transcriptional activity of RAR." provenance.