Matches in Nanopublications for { ?s <http://www.w3.org/ns/prov#value> ?o ?g. }
- _5 value "Modified assertion" provenance.
- _3 value "Significantly, inhibition of p53-mediated transcriptional repression with TSA markedly inhibits apoptosis induction by p53." provenance.
- _4 value "We report that trichostatin A (TSA), an inhibitor of histone deacetylases (HDACs), abrogates the ability of p53 to repress the transcription of two genes that it negatively regulates, Map4 and stathmin." provenance.
- _5 value "IKKbeta efficiently phosphorylated the same Ser residue of p65 in vitro" provenance.
- _7 value "Thus, the leucine zipper domain enables c-Cbl to homodimerize, and homodimerization influences Cbl's signaling function, modulating the activity of Cbl itself and/or affecting Cbl's associations with other signaling proteins in the cell.In cells, the deletion of the leucine zipper caused a decrease in both the tyrosine phosphorylation of Cbl and its association with the epidermal growth factor receptor following stimulation with epidermal growth factor, thus demonstrating a role for the leucine zipper in c-Cbl's signaling functions." provenance.
- _8 value "Overexpression of full-length JSAP1 in COS-7 cells led to a considerable enhancement of JNK3 activation, and modest enhancement of JNK1 and JNK2 activation, by the MEKK1-SEK1 pathway" provenance.
- _6 value "Furthermore, HDAC4 and MEF2C individually upregulate but together downmodulate c-jun promoter activity." provenance.
- _5 value "Modified assertion" provenance.
- _3 value "In addition, in recent in vivo studies, targeted disruption of the genes for MCP-1, CCR2, and CXCR2 significantly decreased atherosclerotic lesion formation and lipid deposition when the disrupted gene was bred or transferred into mouse strains prone to develop atherosclerotic-like lesions (36, 37, 29)." provenance.
- _4 value "Western blot analysis for IP-10, Mig, and I-TAC extended Northern blot results to detection of all 3 proteins after IFN-g stimulation in ECs (Figure 7b)." provenance.
- _4 value "the glucocorticoid receptor (GR) is suggested to mediate MP actions" provenance.
- _5 value "Here we report the isolation of a gene, LFG, that protects cells uniquely from Fas" provenance.
- _3 value "The injured and elastin peptide, val-gly-val-ala-pro-gly, treated VSMC synthesized active MMPs and reduced expression of TIMP." provenance.
- _3 value "Cyclic AMP strongly inhibited cdk1 and cdk2 expression." provenance.
- _3 value "Cyclic AMP strongly inhibited cdk1 and cdk2 expression." provenance.
- _3 value "Insulin inhibited the stimulatory effect of a high level of glucose (25 mM) and phorbol 12-myristate 13-acetate, an activator of protein kinase C) on the secretion of ANG and the expression of the ANG messenger RNA in IRPTC." provenance.
- _3 value "Insulin inhibited the stimulatory effect of a high level of glucose (25 mM) and phorbol 12-myristate 13-acetate, an activator of protein kinase C) on the secretion of ANG and the expression of the ANG messenger RNA in IRPTC." provenance.
- _3 value "Okadaic acid and calyculin A had no effect on pRB phosphatase activity even though PP2A was completely inhibited. The decrease in pRB phosphorylation correlated with inhibitor-induced suppression of G(1) cyclin-dependent kinases including CDK2, CDK4, and CDK6. The inhibitors also caused decreases in the levels of cyclin D2 and cyclin E, and induction of the cyclin-dependent kinase inhibitors p21(Cip1) and p27(Kip1). " provenance.
- _6 value "Because Raf binds tightly to the GTP-bound form of Ras but not to the GDP-bound form, such differential affinity can be used to determine the GTP-bound status of Ras. " provenance.
- _6 value "DOC-2/DAB2 and DIP1/2 complex represent a negative feedback machinery for several exogenous stimuli-elicited signal cascade. PKC can phosphorylate the N-terminal domain of DOC-2/DAB2 (serine 24) that recruits DIP1/2 to inactivate Ras protein. On the other hand, shortly after the treatment of peptide growth factors, the C terminus of DOC-2/DAB2 (proline-rich domain) competes with SOS for Grb 2 binding, which leads to the inactivation of the MAP pathway. " provenance.
- _6 value "DOC-2/DAB2 and DIP1/2 complex represent a negative feedback machinery for several exogenous stimuli-elicited signal cascade. PKC can phosphorylate the N-terminal domain of DOC-2/DAB2 (serine 24) that recruits DIP1/2 to inactivate Ras protein. On the other hand, shortly after the treatment of peptide growth factors, the C terminus of DOC-2/DAB2 (proline-rich domain) competes with SOS for Grb 2 binding, which leads to the inactivation of the MAP pathway. " provenance.
- _6 value "DOC-2/DAB2 and DIP1/2 complex represent a negative feedback machinery for several exogenous stimuli-elicited signal cascade. PKC can phosphorylate the N-terminal domain of DOC-2/DAB2 (serine 24) that recruits DIP1/2 to inactivate Ras protein. On the other hand, shortly after the treatment of peptide growth factors, the C terminus of DOC-2/DAB2 (proline-rich domain) competes with SOS for Grb 2 binding, which leads to the inactivation of the MAP pathway. " provenance.
- _5 value "DOC-2/DAB2, a novel phosphoprotein with signal-transducing capability, inhibits human prostatic cancer cells (Tseng, C.-P., Ely, B. D., Li, Y., Pong, R.-C., and Hsieh, J.-T. (1998) Endocrinology 139, 3542-3553). However, its mechanism of action is not understood completely. This study delineates the functional significance of DOC-2/DAB2 protein phosphorylation and demonstrates that in vivo activation of protein kinase C (PKC) by 12-O-tetradecanoylphorbol-13-acetate (TPA) induces DOC-2/DAB2 phosphorylation, including a serine residue at position 24. Mutation of Ser(24) to Ala reduced DOC-2/DAB2 phosphorylation by PKC. Using a synthetic Ser(24) peptide (APS(24)KKEKKKGSEKTD) or recombinant DOC-2/DAB2 as substrates, PKCbetaII, PKCgamma, and PKCdelta (but not casein kinase II) directly phosphorylated Ser(24) in vitro. This indicates that DOC-2/DAB2 is a PKC-specific substrate. Since expression of wild-type DOC-2/DAB2, but not the S24A mutant, inhibited TPA-induced AP-1 activity in prostatic epithelial cells, phosphorylation of Ser(24) appears to play a critical role in modulating TPA-induced AP-1 activity. Taken together, these data suggest that PKC-regulated phosphorylation of DOC-2/DAB2 protein may help its growth inhibitory function." provenance.
- _5 value "In LPS-injected PPARa 1/1 mice, treatment with fenofibrate decreased significantly IL-6 mRNA levels in aortas, whereas fenofibrate did not have any effect in PPARa 2/2 mice (Fig. 1B)." provenance.
- _5 value "LPS stimulation resulted in a significant increase of IL-6 production (approximately 3-fold) in wild-type mice aortas, in agreement with previous observations (26). However, this increase was much greater in aortas isolated from the PPARa 2/2 mice (12-fold, p , 0.03)." provenance.
- _3 value "We and others (19, 20) reported that fibrates decrease plasma concentrations of inflammatory cytokines, such as IL-6 and tumor necrosis factor a, in human patients with angiographically established atherosclerosis and prevent the induction of IL-6 production by IL-1b in SMC." provenance.
- _4 value "Angiotensin II induces the tyrosine and serine phosphorylation of Stat1 and Stat3. Under High Glucose condition both the extent and the duration of phosphorylation of Stat1 and Stat3 was increased." provenance.
- _3 value "In summary, lovastatin and simvastatin predominantly decrease the expression of the glycoprotein thrombospondin-1, which is functionally associated with smooth muscle cell migration and proliferation." provenance.
- _6 value "Overexpression of MKP-1 in DU145 cells blocked activation of stress-activated protein kinase (SAPK/JNK)." provenance.
- _4 value "retinoic acid also promotes ucp1 gene transcription and its structure makes it a possible activator of UCP1, we hypothesized that retinoic acid, like noradrenaline, could have a dual action and trigger the activity of the protein UCP1 itself.Here we show that retinoic acid strongly increases proton transport by UCP1 in brown adipose tissue mitochondria" provenance.
- _3 value "GGT expression was up-regulated on peripheral blood T cells following activation in vitro by either superantigen, phorbol ester, or IL-15" provenance.
- _3 value "Mid-to-late events included progressive and sustained increases in p27 and p16 protein expression, a decrease in retinoblastoma protein expression, and an increase in the transcription factor E2F1" provenance.
- _5 value "Both SOCS-1 and SOCS-3 were able to inhibit GH-induced STAT5 (signal transducer and activator of transcription) activation. SOCS-1 inhibited the tyrosine kinase activity of Janus kinase 2 (JAK2) directly" provenance.
- _3 value "Activation of MAP kinase by phorbol ester treatment caused a 3-5-fold reduction in apoCIII transcription" provenance.
- _6 value "The major protein binding to this site was identified as the nuclear hormone receptor HNF4. An increase in HNF4 mRNA and protein levels was observed in HepG2 cells after treatment with PD98059, indicating that the MAP kinase pathway regulates the expression of the HNF4 gene." provenance.
- _6 value "Cdk2 activity was inhibited by the addition of 400 nM of recombinant GST-p21Cip1. When SET was added together with p21Cip1 it reversed the inhibitory effect of p21Cip1 on Cyclin E-Cdk2 activity." provenance.
- _4 value "These experiments revealed that Fn14 expression decreased cellular adhesion to the extracellular matrix proteins fibronectin and vitronectin and also reduced serum-stimulated cell growth and migration" provenance.
- _2 value "the proinflammatory products of activated monocytes and macrophages (e.g., TNF and NO) can also injure normal cells." provenance.
- _4 value "Modified assertion" provenance.
- _3 value "These results indicate that cisplatin enhances adenosine A(1) receptor expression in the rat testis, possibly through promotion of oxidative stress." provenance.
- _3 value "trabeculin is ubiquitously expressed and is distributed throughout the cytoplasm, though the protein was found to be greatly up-regulated upon differentiation of myoblasts into myotubes" provenance.
- _5 value "Kinase-defective point and deletion variants of RIP2 also significantly blocked the activation of ERK2 by TNFalpha but not epidermal growth factor." provenance.
- _6 value "RIP2 in turn is activated through its interaction with Ras-activated Raf1." provenance.
- _5 value "Here we show that cyclic-AMP-dependent protein kinase (PKA) phosphorylates serine residue 23 in the KIM of HePTP in vitro and in intact cells." provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _7 value "This modification reduces binding of MAP kinases to the KIM, an effect that is prevented by mutation of serine 23 to alanine." provenance.
- _7 value "This modification reduces binding of MAP kinases to the KIM, an effect that is prevented by mutation of serine 23 to alanine." provenance.
- _7 value "This modification reduces binding of MAP kinases to the KIM, an effect that is prevented by mutation of serine 23 to alanine." provenance.
- _5 value "Whole cell extract of wild-type K562 cells, a transformant cell line of K562 expressing HSF2 and HeLa cells expressing HSF2 transfected with the reporter construct of Ccta gene was found to bind the HSE sequence of Ccta gene (Tcp1), thus activating the Cct gene transcription." provenance.
- _5 value "Whole cell extract of wild-type K562 cells, a transformant cell line of K562 expressing HSF2 and HeLa cells expressing HSF2 transfected with the reporter construct of Ccte gene was found to bind the HSE sequence of Ccte gene, thus activating the Cct gene transcription." provenance.
- _5 value "Whole cell extract of wild-type K562 cells, a transformant cell line of K562 expressing HSF2 and HeLa cells expressing HSF2 transfected with the reporter construct of Cctz-1 gene was found to bind the HSE sequence of Cctz-1 gene, thus activating the Cct gene transcription." provenance.
- _4 value ". IL-18 independently promoted GM-CSF and nitric oxide production , and it induced significant TNF-alpha synthesis by CD14(+) macrophages in synovial cultures; the latter effect was potentiated by IL-12 or IL-15" provenance.
- _4 value ". IL-18 independently promoted GM-CSF and nitric oxide production , and it induced significant TNF-alpha synthesis by CD14(+) macrophages in synovial cultures; the latter effect was potentiated by IL-12 or IL-15" provenance.
- _8 value ". IL-18 independently promoted GM-CSF and nitric oxide production , and it induced significant TNF-alpha synthesis by CD14(+) macrophages in synovial cultures; the latter effect was potentiated by IL-12 or IL-15" provenance.
- _6 value "IL-18 and IL-12 alone induced low levels of IFN- gamma synthesis by cultures of either SF mononuclear cells or synovial membrane?derived single-cell suspensions (Figure 4)" provenance.
- _4 value "TNF-alpha and IFN-gamma synthesis was suppressed by IL-10 and TGF-beta" provenance.
- _4 value "TNF-alpha and IFN-gamma synthesis was suppressed by IL-10 and TGF-beta" provenance.
- _5 value "The silencing activity of TIF1alpha appears to result chiefly from histone deacetylation, whereas that of TIF1beta may be mediated via both HP1 binding and histone deacetylation." provenance.
- _5 value "The silencing activity of TIF1alpha appears to result chiefly from histone deacetylation, whereas that of TIF1beta may be mediated via both HP1 binding and histone deacetylation." provenance.
- _3 value "The silencing activity of TIF1alpha appears to result chiefly from histone deacetylation, whereas that of TIF1beta may be mediated via both HP1 binding and histone deacetylation." provenance.
- _3 value "The silencing activity of TIF1alpha appears to result chiefly from histone deacetylation, whereas that of TIF1beta may be mediated via both HP1 binding and histone deacetylation." provenance.
- _3 value "The silencing activity of TIF1alpha appears to result chiefly from histone deacetylation, whereas that of TIF1beta may be mediated via both HP1 binding and histone deacetylation." provenance.
- _6 value "The silencing activity of TIF1alpha appears to result chiefly from histone deacetylation, whereas that of TIF1beta may be mediated via both HP1 binding and histone deacetylation." provenance.
- _5 value "Here, we identified the HMG box containing protein-1 (HBP1) as a factor binding to HSS3 of the hCD2 LCR" provenance.
- _4 value "IFN-beta1A enhanced the production of IL4 in MBP-reactive T-cell clones obtained from patients with Multiple sclerosis. IL-4 increased from 42 to 129 pg/ml. IFN-beta1a also enhanced the production of IL4 in Myelin basic protein (MBP)-primed PBMC culture." provenance.
- _6 value "Modified assertion" provenance.
- _3 value "In contrast, 24-h cAMP inhibited PAI-1 gene expression and protein production under basal conditions and in the presence of dexamethasone." provenance.
- _3 value "To understand the molecular processes of continuous vasospasm of cerebral arteries after subarachnoid hemorrhage, mRNA differential display and screening of cDNA expression array were performed to identify genes that are differentially expressed in vasospastic arteries of canine two-hemorrhage models Cerebral vasospasm after subarachnoid hemorrhage, which is the most serious complication causing cerebral ischemia in patients, is a complex pathologic process characterized by continuous contraction of arterial smooth muscle for several weeks" provenance.
- _3 value "To understand the molecular processes of continuous vasospasm of cerebral arteries after subarachnoid hemorrhage, mRNA differential display and screening of cDNA expression array were performed to identify genes that are differentially expressed in vasospastic arteries of canine two-hemorrhage models Cerebral vasospasm after subarachnoid hemorrhage, which is the most serious complication causing cerebral ischemia in patients, is a complex pathologic process characterized by continuous contraction of arterial smooth muscle for several weeks" provenance.
- _3 value "To understand the molecular processes of continuous vasospasm of cerebral arteries after subarachnoid hemorrhage, mRNA differential display and screening of cDNA expression array were performed to identify genes that are differentially expressed in vasospastic arteries of canine two-hemorrhage models Cerebral vasospasm after subarachnoid hemorrhage, which is the most serious complication causing cerebral ischemia in patients, is a complex pathologic process characterized by continuous contraction of arterial smooth muscle for several weeks" provenance.
- _3 value "To understand the molecular processes of continuous vasospasm of cerebral arteries after subarachnoid hemorrhage, mRNA differential display and screening of cDNA expression array were performed to identify genes that are differentially expressed in vasospastic arteries of canine two-hemorrhage models Cerebral vasospasm after subarachnoid hemorrhage, which is the most serious complication causing cerebral ischemia in patients, is a complex pathologic process characterized by continuous contraction of arterial smooth muscle for several weeks" provenance.
- _3 value "To understand the molecular processes of continuous vasospasm of cerebral arteries after subarachnoid hemorrhage, mRNA differential display and screening of cDNA expression array were performed to identify genes that are differentially expressed in vasospastic arteries of canine two-hemorrhage models Cerebral vasospasm after subarachnoid hemorrhage, which is the most serious complication causing cerebral ischemia in patients, is a complex pathologic process characterized by continuous contraction of arterial smooth muscle for several weeks" provenance.
- _3 value "To understand the molecular processes of continuous vasospasm of cerebral arteries after subarachnoid hemorrhage, mRNA differential display and screening of cDNA expression array were performed to identify genes that are differentially expressed in vasospastic arteries of canine two-hemorrhage models Cerebral vasospasm after subarachnoid hemorrhage, which is the most serious complication causing cerebral ischemia in patients, is a complex pathologic process characterized by continuous contraction of arterial smooth muscle for several weeks" provenance.
- _3 value "In addition, overexpression of a constitutively active protein kinase B increased p85alphaPI 3-K mRNA levels. These results indicate that the phosphatidylinositol 3-kinase/PKB/p70S6 kinase pathway is required for the stimulation by insulin of p85alphaPI 3-K gene expression in human muscle cells." provenance.
- _4 value "Insulin acutely up-regulates p85alpha phosphatidylinositol 3-kinase (p85alphaPI 3-K) mRNA levels in human skeletal muscle" provenance.
- _3 value "Three highly homologous acyl-CoA thioesterase genes, Cte1, Mte1, and Pte1, are located within a narrow cluster on chromosone 12. All four thioesterases are differentially expressed in tissues examined, but all are inducible at mRNA level by treatment with the peroxisome proliferator clofibrate, or during the physiological condition of fasting, both conditions causing a perturbation in overall lipid homeostasis." provenance.
- _4 value "DOKL and p62(dok) bind through their PTB domains to the Abelson tyrosine kinase in a kinase-dependent manner in both yeast and mammalian cells. DOKL is phosphorylated by the Abl tyrosine kinase in vivo. In contrast to p62(dok), DOKL lacks YxxP motifs in the C terminus and does not bind to Ras GTPase-activating protein (RasGAP) upon phosphorylation. Overexpression of DOKL, but not p62(dok), suppresses v-Abl-induced mitogen-activated protein (MAP) kinase activation but has no effect on constitutively activated Ras- and epidermal growth factor-induced MAP kinase activation. T" provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _5 value "deltaEF1 has a second mechanism of transrepression recruiting CtBP1 or CtBP2 as its corepressor" provenance.
- _3 value "We found, that histamine inhibited the LPS induced transcription of IFNgamma gene and biosynthesis of IFNgamma protein in PMBC" provenance.
- _6 value "Culture of lymphocytes in the presence of IL-12 induced a marked increase in the amounts of MIP-1alpha, MIP-1beta and RANTES in the culture supernatants." provenance.
- _3 value "Concomitantly, exposure of AEC to hypoxia (18 h 0% O(2)) induced a 3-fold increase of glucose transporter GLUT1 at both protein and messenger RNA (mRNA) levels." provenance.
- _5 value "LPL dose-dependently increased the binding of native LDL to both versican and biglycan" provenance.
- _5 value "the addition of LPL to oxidized LDL together with biglycan or versican allowed the oxidized LDL to bind the proteoglycans in an LPL dose-dependent manner. Addition of LPL had a greater relative effect on the binding of extensively oxidized LDL to proteoglycans compared with native LDL" provenance.
- _5 value "The results show that Lyn phosphorylates SHPTP1 at the C-terminal Tyr-564 site. Lyn-mediated phosphorylation of SHPTP1 stimulates SHPTP1 tyrosine phosphatase activity." provenance.
- _6 value "The results show that Lyn phosphorylates SHPTP1 at the C-terminal Tyr-564 site. Lyn-mediated phosphorylation of SHPTP1 stimulates SHPTP1 tyrosine phosphatase activity." provenance.
- _4 value "Furthermore, insulin-stimulated activation of Akt, but not mitogen-activated protein kinase, was diminished." provenance.
- _5 value "Insulin-induced tyrosine phosphorylation of the insulin receptor, insulin receptor substrate 1 (IRS-1), and IRS-2 was reduced by prestimulation of beta(3)-adrenergic receptors (CL316243)" provenance.
- _3 value "Similarly, insulin-induced IRS-1-associated and phosphotyrosine-associated phosphatidylinositol 3-kinase (PI 3-kinase) activity, but not IRS-2-associated PI 3-kinase activity, was reduced by beta(3)-adrenergic prestimulation" provenance.
- _5 value "These effects appear to be protein kinase A-dependent. Furthermore inhibition of protein kinase C restored the beta(3)-receptor-mediated reductions in insulin-induced IRS-1 tyrosine phosphorylation and IRS-1-associated PI3-kinase activity" provenance.
- _6 value "These effects appear to be protein kinase A-dependent. Furthermore inhibition of protein kinase C restored the beta(3)-receptor-mediated reductions in insulin-induced IRS-1 tyrosine phosphorylation and IRS-1-associated PI3-kinase activity" provenance.
- _6 value "These effects appear to be protein kinase A-dependent. Furthermore inhibition of protein kinase C restored the beta(3)-receptor-mediated reductions in insulin-induced IRS-1 tyrosine phosphorylation and IRS-1-associated PI3-kinase activity" provenance.
- _5 value "These effects appear to be protein kinase A-dependent. Furthermore inhibition of protein kinase C restored the beta(3)-receptor-mediated reductions in insulin-induced IRS-1 tyrosine phosphorylation and IRS-1-associated PI3-kinase activity" provenance.
- _5 value "treatment of the interphase cells with calyculin A revealed phosphorylation at all the three sites of desmin. We also found that an antibody, which specifically recognizes vimentin phosphorylated at Ser-71 by Rho kinase, became immunoreactive after calyculin A treatment." provenance.
- _4 value "A Binding Site for the Transcription Factor Grainyhead/Nuclear Transcription Factor-1 Contributes to Regulation of the Drosophila Proliferating Cell Nuclear Antigen Gene Promoter * Yuko Hayashi , Masahiro Yamagishi , Yoshio Nishimoto , Osamu Taguchi , Akio Matsukage , and Masamitsu Yamaguchi || From the Laboratory of Cell Biology and the Laboratory of Experimental Pathology, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya, 464-8681, Japan ABSTRACT TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES" provenance.
- _5 value "Fas (CD95 or APO-1), a transmembrane cell surface receptor of the tumor necrosis factor receptor family, is up-regulated in activated T lymphocytes. Our present study identified an upstream enhancer element (between nucleotide positions -862 and -682) containing a GA-binding protein (GABP) site and a low affinity activating protein-1 (AP-1)-binding site. T cell activation increased the DNA binding of GABP and AP-1 to this enhancer site." provenance.
- _3 value "We further show that anti-CD3 mAb, phorbol 12-myristate 13-acetate, and phorbol 12-myristate 13-acetate/ionomycin strongly activated promoters carrying multiple copies of the Fas enhancer, and mutation of either the GABP or AP-1 binding site severely reduced transcriptional activity." provenance.
- _6 value "Modified assertion" provenance.
- _4 value "Scavenger receptors bind and internalize modified low-density lipoprotein (LDL) and high-density lipoprotein (HDL). Because the expression of scavenger receptors is not down-regulated by cholesterol, macrophages (Mphi) expressing scavenger receptors can internalize substantial quantities of cholesteryl ester from oxidized LDL and HDL, leading to foam cell formation" provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.