Matches in Nanopublications for { ?s <http://www.w3.org/ns/prov#value> ?o ?g. }
- _3 value "We have used cDNA arrays to analyze differential gene expression in primary cultures of human melanocytes in response to 365-nm UVA. Among 588 genes studied, 11 were overexpressed. These genes included genes involved in cell cycle regulation (GADD45, CIP1/WAF1), in stress response (HSP70, HSP40, HSP86), in apoptosis (GADD153, tristetraproline) and genes encoding transcription factors (EGR-1, ETR-101, c-JUN, ATF4)" provenance.
- _3 value "We have used cDNA arrays to analyze differential gene expression in primary cultures of human melanocytes in response to 365-nm UVA. Among 588 genes studied, 11 were overexpressed. These genes included genes involved in cell cycle regulation (GADD45, CIP1/WAF1), in stress response (HSP70, HSP40, HSP86), in apoptosis (GADD153, tristetraproline) and genes encoding transcription factors (EGR-1, ETR-101, c-JUN, ATF4)" provenance.
- _3 value "PROG triggered a quick (visible as soon as 15 min), strong (6 to 18-fold) and transient (1-2 h) stimulation of Egr-1, Egr-2, Egr-3 and Fos B genes expression" provenance.
- _6 value "The GnRHR is also expressed in non-pituitary cells and was found to be involved in the inhibition of cell proliferation in certain cells. Therefore, GnRHR represents a potential target for GnRH-analogs used for cancer treatment. Interestingly, the signaling mechanism of the GnRHR in other cell types significantly differs from that in pituitary cells. Studies conducted in GnRHR-expressing COS7 cells have shown that GnRHR transmits its signals mainly via Gi, EGF receptor, c-Src, and is not dependent on PKC." provenance.
- _6 value "The GnRHR is also expressed in non-pituitary cells and was found to be involved in the inhibition of cell proliferation in certain cells. Therefore, GnRHR represents a potential target for GnRH-analogs used for cancer treatment. Interestingly, the signaling mechanism of the GnRHR in other cell types significantly differs from that in pituitary cells. Studies conducted in GnRHR-expressing COS7 cells have shown that GnRHR transmits its signals mainly via Gi, EGF receptor, c-Src, and is not dependent on PKC." provenance.
- _7 value "Page 54 - \"Crk appears to bind Gab1 in response to Met but not other receptor tyrosine kinases.\"" provenance.
- _5 value "Page 54 - \"HGF/SF stimulation promotes the tyrosine phosphorylation of b-catenin, interfering with the ability of b-catenin to bind the intracellular portion of E-cadherin (Figure 6). The result is a dismantling of cell-cell adhesion complexes (105).\"" provenance.
- _6 value "Page 54 - \"HGF/SF-stimulated motility of hepatocellular carcinoma (HCC) cell lines is associated with activation of PI3-K. Wortmannin has been shown to prevent migration not only in these cells but also primary HCC cells (103).\"" provenance.
- _4 value "Human airway epithelial cells were cultured for 4 hours in the presence of 50 ng/ml IL-1beta, TNF-alpha and IFN-gamma (cytomix). There was a significant decrease in the level of iNOS mRNA expression in epithelial cells derived from asthmatic patients compared to cells from normal subjects." provenance.
- _6 value "Unliganded PR-B disrupted the DNA-binding activity of BTEB1 in gel retardation assays, and this effect was enhanced by the presence of PR ligand." provenance.
- _3 value "After adrenalectomy, we observed increased mRNA levels for SPC1, SPC3, SPC4, and SPC6, but not for SPC2, in POMC-positiveanterior pituitary cells." provenance.
- _3 value "After adrenalectomy, we observed increased mRNA levels for SPC1, SPC3, SPC4, and SPC6, but not for SPC2, in POMC-positiveanterior pituitary cells." provenance.
- _6 value "These increased levels were reversed by dexamethasone treatment." provenance.
- _5 value "Activation of AR NTD by IL-6 was suppressed by STAT3 dominant negative mutants. STAT3 in a dimer form interacts with aminoacids 234-558 of the AR NTD in nucleus." provenance.
- _10 value "PKCa Associates with b1 Integrin and CADTK...We therefore next determined whether PKCa and b1 integrin are associated in MM cells. Constitutive complex formation between these two proteins was demonstrated by co-immunoprecipitation...Upon activation and translocation, conventional PKCs associate with proteins of the transmembrane-4 superfamily (TM4SF or tetraspans) linking PKC to several subsets of integrins (38) including a4b1 (39) and a5b1...the integrin-TM4SF-PKC complex formation results in phosphorylation of the integrin alpha cytoplasmic tail" provenance.
- _3 value "b1 integrin neutralizing mAb (but not irrelevant IgG) mediated dose-dependent inhibition of VEGF-triggered MM cell migration...These data confirm that b1 integrin (CD29) is the integrin primarily associated with VEGF-triggeredMMcell migration on fibronectin." provenance.
- _5 value "b1 integrin neutralizing mAb (but not irrelevant IgG) mediated dose-dependent inhibition of VEGF-triggered MM cell migration...These data confirm that b1 integrin (CD29) is the integrin primarily associated with VEGF-triggeredMMcell migration on fibronectin." provenance.
- _3 value "We also show here that CD40 expression in lipopolysaccharide-stimulated cells is up-regulated by NF-kappaB through two distinct sites. One of these sites (-128 to -119) was shown to bind p50 and p65 members of the NF-kappaB family, while the other site (-562 to -553) bound only p65. Transfectants of p65 were generated using RAW 264 cells, and it was shown that the up-regulation of CD40 mRNA expression was dependent on the presence of the p65 molecule." provenance.
- _4 value "Our results indicate that BCR-ABL regulates the expression of C/EBPalpha by inducing hnRNP E2-which inhibits the translation of CEBPA mRNA." provenance.
- _5 value "Recently, it has been described that STATs (STAT 1, 3 and 5) are constitutively tyrosine phosphorylated and activated in AML blasts," provenance.
- _6 value "Modified assertion" provenance.
- _5 value "Similarly, ribosomal protein S6 kinase 2 (S6K2), a recently identified homologue of S6K1 was activated by FGF-2 in H-510, but not H-69 cells" provenance.
- _4 value "Treatment of MIF (10 microg/ml) led to phosphorylation of multiple cellular proteins of 60, 34 and 30 kDa in comparison with the control and the phosphorylated 60 kDa protein was considered to be an autophosphorylated member of the Src tyrosine kinases and autophosphorylation was enhanced at 3 minutes after stimulation but the protein level was sustained up to 1 hour. Further, treatment of PP2, a selective inhibitor of Src kinases markedly suppressed the upregulation of MMP13 induced by MIF. Thus, MIF enhanced the kinase activity but protein level was not increased." provenance.
- _4 value "Treatment of MIF (10 microg/ml) led to phosphorylation of multiple cellular proteins of 60, 34 and 30 kDa in comparison with the control and the phosphorylated 60 kDa protein was considered to be an autophosphorylated member of the Src tyrosine kinases and autophosphorylation was enhanced at 3 minutes after stimulation but the protein level was sustained up to 1 hour. Further, treatment of PP2, a selective inhibitor of Src kinases markedly suppressed the upregulation of MMP13 induced by MIF. Thus, MIF enhanced the kinase activity but protein level was not increased." provenance.
- _5 value "Treatment of MIF (10 microg/ml) led to phosphorylation of multiple cellular proteins of 60, 34 and 30 kDa in comparison with the control and the phosphorylated 60 kDa protein was considered to be an autophosphorylated member of the Src tyrosine kinases and autophosphorylation was enhanced at 3 minutes after stimulation but the protein level was sustained up to 1 hour. Further, treatment of PP2, a selective inhibitor of Src kinases markedly suppressed the upregulation of MMP13 induced by MIF. Thus, MIF enhanced the kinase activity but protein level was not increased." provenance.
- _3 value "Since Grp94 is known to inhibit apoptosis by maintaining intracellular Ca(2+) homeostasis" provenance.
- _6 value "Here, we report that p75 activates Rac GTPase, which in turn activates c-jun N-terminal kinase (JNK), including an injury-specific JNK3, in an NGF-dependent manner." provenance.
- _6 value "The neurotrophin receptor p75 can induce apoptosis both in vitro and in vivo p75 activates Rac GTPase, which in turn activates c-jun N-terminal kinase (JNK), including an injury-specific JNK3, in an NGF-dependent manner" provenance.
- _3 value "Modified assertion" provenance.
- _5 value "Modified assertion" provenance.
- _4 value "Results demonstrate that ischemia increases myocardial aldose reductase activity and that these increases are, in part, due to activation by nitric oxide" provenance.
- _6 value "activin A induced phosphorylation of Smad 2, and BMP-7 induced Smad 1, both of which were inhibited by follistatin" provenance.
- _5 value "# Ariadne: First, we showed that the expression of transgelin is abolished at the level of transcription by oncogenic Ras in NIH 3T3 mouse fibroblasts, RIE-1 rat intestinal epithelial cells, and HT1080 human fibrosarcoma cells. [Expression] # Ariadne: We further characterized transgelin as a gene whose expression was abolished by Ras. [Regulation]" provenance.
- _3 value "CTGF is able to promote VSMC proliferation and migration and influences the composition of extracellular matrix." provenance.
- _6 value "Evidence is provided that attenuation of Erk-1/-2 activity by estrogen occurs via GPR30-dependent stimulation of adenylyl cyclase and cAMP-dependent signaling that results in Raf-1 inactivation." provenance.
- _5 value "AR and ERalpha transcriptional activities were inhibited by PAK6 in transient transfections with episomal and integrated reporter genes." provenance.
- _4 value "Blockage of NFkappaB transactivation activity abrogated IL-1beta-induced promatrilysin expression to baseline levels suggesting that NFkappaB transactivation activity is necessary. These data provide evidence that NFkappaB-mediated IL-6 synthesis is required for IL-1beta-induced promatrilysin expression, and IL-6 signaling through STAT3 plays a role in IL-1beta-induced promatrilysin expression." provenance.
- _3 value "The expression of brain-derived neurotrophic factor (BDNF) mRNA is induced by neuronal activity through increased intracellular calcium." provenance.
- _3 value "% Phosphorylation of 4E-BP1 dissociates it from eIF-4E, relieving the translation block. In the present study we demonstrated that UVB induced 4E-BP1 phosphorylation at multiple sites, Thr-36, Thr-45, Ser-64, and Thr-69, leading to dissociation of 4E-BP1 from eIF-4E." provenance.
- _5 value "An analysis of endogenous BCL-X(L) levels in AFX-expressing cells revealed enhanced down-regulation of the transcript ( approximately 1.3-1.7-fold) and protein, and BCL-6 directly binds to and suppresses the BCL-X(L) promoter." provenance.
- _6 value "Modified assertion" provenance.
- _4 value "Although the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors cerivastatin, fluvastatin, and pitavastatin were not ligands for these three nuclear receptors in the CARLA, they induced transcriptional activation of PPARalpha/RXRalpha, PPARdelta/RXRalpha, and PPARgamma2/RXRalpha." provenance.
- _6 value "Although the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors cerivastatin, fluvastatin, and pitavastatin were not ligands for these three nuclear receptors in the CARLA, they induced transcriptional activation of PPARalpha/RXRalpha, PPARdelta/RXRalpha, and PPARgamma2/RXRalpha." provenance.
- _6 value "Although the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors cerivastatin, fluvastatin, and pitavastatin were not ligands for these three nuclear receptors in the CARLA, they induced transcriptional activation of PPARalpha/RXRalpha, PPARdelta/RXRalpha, and PPARgamma2/RXRalpha." provenance.
- _4 value "Although the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors cerivastatin, fluvastatin, and pitavastatin were not ligands for these three nuclear receptors in the CARLA, they induced transcriptional activation of PPARalpha/RXRalpha, PPARdelta/RXRalpha, and PPARgamma2/RXRalpha." provenance.
- _6 value "Although the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors cerivastatin, fluvastatin, and pitavastatin were not ligands for these three nuclear receptors in the CARLA, they induced transcriptional activation of PPARalpha/RXRalpha, PPARdelta/RXRalpha, and PPARgamma2/RXRalpha." provenance.
- _6 value "We found that bezafibrate was a ligand for PPARalpha, delta, and gamma in the CARLA and that bezafibrate induced transcriptional activation of PPARalpha/RXRalpha, PPARdelta/RXRalpha, and PPARgamma/RXRalpha." provenance.
- _6 value "We found that bezafibrate was a ligand for PPARalpha, delta, and gamma in the CARLA and that bezafibrate induced transcriptional activation of PPARalpha/RXRalpha, PPARdelta/RXRalpha, and PPARgamma/RXRalpha." provenance.
- _7 value "Pge2 activates gp130 by promoting its dimerization" provenance.
- _3 value "Pge2 biosynthesis by Cox2 plays a pivotal role in inflammation and carcinogenesis hypothesized that increased expression of Cox2, with resultant increased levels of Pge2 in human PIN cells, activates the Il6 signaling pathway" provenance.
- _2 value "Pge2 stimulates soluble Il6 receptor release, gp130 dimerization, Stat3 protein phosphorylation, and DNA binding activity these events, induced by Pge2, lead to increased PIN cell growth" provenance.
- _5 value "Pge2 stimulates soluble Il6 receptor release, gp130 dimerization, Stat3 protein phosphorylation, and DNA binding activity these events, induced by Pge2, lead to increased PIN cell growth" provenance.
- _4 value "Pge2 stimulates soluble Il6 receptor release, gp130 dimerization, Stat3 protein phosphorylation, and DNA binding activity these events, induced by Pge2, lead to increased PIN cell growth" provenance.
- _2 value "increased biosynthesis of prostaglandin E2 [Pge2], a major eicosanoid product of the Cox2-catalyzed reaction, plays a pivotal role in inflammation" provenance.
- _5 value "Rho, Rac, and Cdc42 elicit distinct morphological changes in cultured mammalian cells (Hall, 1998). Specifically, Rho activation induces stress fiber formation. We found that N-Daam1 disrupts, whereas C-Daam1 enhances, the formation of actin stress fibers (Figure 4A). Thus, the cell biological assay is consistent with the biochemical evidence that Daam1 regulates RhoA activity." provenance.
- _6 value "Since Daam1 binds to Dvl and RhoA, we tested whether Daam1 forms a ternary complex with Dvl and RhoA. We immunoprecipitated the endogenous RhoA from 293T cells treated with or without Wnt-1 CM. Strikingly, we found that the endogenous Dvl protein is associated with RhoA only upon Wnt stimulation (Figure 2G)." provenance.
- _5 value "Surprisingly, Wnt-1, which has been studied primarily in ?-catenin signaling, activates RhoA and Rac, but not Cdc42 (Figure 1B)." provenance.
- _5 value "We also employed double-strand (ds) RNA mediated interference (RNAi) (Elbashir et al., 2001) to deplete endogenous Daam1 protein. The ds RNAi oligo for Daam1 reduces the protein level of Daam1, but not of RhoA, ?-catenin (Figure 3C) or Dvl2 (not shown). Importantly, the RNAi oligo inhibits RhoA activation by Wnt-1, Fz, or Dvl, but not by Ephexin (Figure 3C). A control ds RNAi oligo has no effect on Daam1 or RhoA protein level or RhoA activation (Figure 3C). Thus, Daam1 is essential for Wnt/Fz/Dvl activation of RhoA. AN ACTIVATED DAAM1 INDUCES RHO ACTIVATION IN A RHO-GEF-DEPENDENT MANNER Previous studies suggest that deletion of the amino terminal domain results in constitutive activation of FH proteins by releasing an intramolecular inhibition (Watanabe et al. 1999; Tominaga et al. 2000 and Westendorf 2001). C-Daam1 thus may represent an activated form of Daam1. Indeed, while the full length Daam1 is inactive, C-Daam1 overexpression causes activation of RhoA, but not of Rac or Cdc42 (Figure 3D). C-Daam1 activation of RhoA is not affected by ?PDZ-Dvl (Figure 3A), which inhibits RhoA activation by Fz or Dvl (Figure 1F), further demonstrating that Daam1 functions downstream of Dvl in Rho activation." provenance.
- _5 value "In various cell types, AKT phosphorylates and inhibits glycogen synthase kinase-3 (GSK-3). Upon serum stimulation of EC, GSK-3beta was phosphorylated at Ser-9." provenance.
- _4 value "Treatment of Fao cells with 50ng/ml NDF-beta1 for 30 min increased the tyrosine phosphorylation of ERBB1, ERBB2 and ERBB3." provenance.
- _4 value "Dominant negative PI-3K-pOPRSVI construct transfected K562 cells were cultured with IPTG, the inducible expression of DN-PI-3K by IPTG was involved in downregulation of CyclinD3, suggesting that PI-3K induces the upregulation of CyclinD3." provenance.
- _5 value "Dominant negative RAS-pOPRSVI construct transfected K562 cells were cultured with IPTG for 24 hours, the inducible expression of DN-RAS by IPTG involved in downregulation of CyclinD3, suggesting that RAS induces the Upregulation of CyclinD3." provenance.
- _4 value "Insulin stimulation resulted in a translocation of PKB beta from cytosol to membranes, and phosphorylation and activation of PKB beta. Phosphoamino acid analysis and phosphopeptide mapping demonstrated that the phosphorylation occurred mainly on serines, also when using calyculin A, and that these were localized within one major phosphopeptide. Radiosequencing showed that the radioactivity was released in Cycle No. 7. In addition, the peptide was specifically immunoprecipitated from a tryptic digest of PKB beta using the anti-phospho-PKB (Ser-473) antibody (From title - Ser-474 is the major target of insulin-mediated phosphorylation of protein kinase B beta in primary rat adipocytes)" provenance.
- _4 value "In addition, over-expression of either Oct-1 or Oct-2 decreased wild-type IL-4 promoter activity, while increasing IL-2 promoter activity.....Thus, the transcription factors, Oct-1 and Oct-2, appear to decrease IL-4 transcription, via interaction with the proximal octamer site located between the IL-4 promoter proximal NFAT binding sites, P0 and P1, while enhancing IL-2 gene transcription." provenance.
- _5 value "In addition, over-expression of either Oct-1 or Oct-2 decreased wild-type IL-4 promoter activity, while increasing IL-2 promoter activity.....Thus, the transcription factors, Oct-1 and Oct-2, appear to decrease IL-4 transcription, via interaction with the proximal octamer site located between the IL-4 promoter proximal NFAT binding sites, P0 and P1, while enhancing IL-2 gene transcription." provenance.
- _5 value "In this work we present evidence that v-Myc encoded by the avian retrovirus MC29 stimulates activating transcription factor 2 (ATF2)-dependent transcription. Analysis of the effect of Myc on ATF2 shows that v-Myc prolongs the half-life of ATF2 and induces the phosphorylation of N-terminal sites of ATF2 (Thr-69 and Thr-71) which have previously been identified as the target sites of stress-activated protein kinases and implicated in the regulation of ATF2 activity." provenance.
- _4 value "To test the hypothesis that changes in the prostanoid profile alter cancer growth, we transduced the retroviral vectors carrying TXA(2) synthase cDNA or PGI(2) synthase cDNA to colon-26 adenocarcinoma cells and subsequently inoculated each transformant to syngeneic BALB/c mice. Tumors derived from TXA(2) synthase transformants grew faster (280%, day 8, versus null-vector control; P < 0.05) and showed more abundant vasculature (204%, versus null-vector control; P < 0.01), whereas tumors from PGI(2) synthase transformants presented opposite effects." provenance.
- _4 value "To test the hypothesis that changes in the prostanoid profile alter cancer growth, we transduced the retroviral vectors carrying TXA(2) synthase cDNA or PGI(2) synthase cDNA to colon-26 adenocarcinoma cells and subsequently inoculated each transformant to syngeneic BALB/c mice. Tumors derived from TXA(2) synthase transformants grew faster (280%, day 8, versus null-vector control; P < 0.05) and showed more abundant vasculature (204%, versus null-vector control; P < 0.01), whereas tumors from PGI(2) synthase transformants presented opposite effects." provenance.
- _4 value "This approach showed that ectopic expression of Dlx2 and Dlx5 induced the expression of glutamic acid decarboxylases (GADs), the enzymes that synthesize GABA." provenance.
- _4 value "We also used this method to show cross-regulation between different Dlx family members. We find that Dlx2 can induce Dlx5 expression, and that Dlx1, Dlx2 and Dlx5 can induce expression from a Dlx5/6-lacZ enhancer/\"reporter construct" provenance.
- _3 value "# Pubmed:omim: BOC, an Ig superfamily member, associates with CDO to positively regulate myogenic differentiation." provenance.
- _5 value "We demonstrate that in T-cell and myoblast cell lines expression of the Nrarp gene is induced by the intracellular domain of the Notch1 protein" provenance.
- _5 value "Modified assertion" provenance.
- _3 value "Summary of cDNAs identified by SSH as being down-regulated in mesangial cells cultured in high glucose" provenance.
- _3 value "Summary of cDNAs identified by SSH as being down-regulated in mesangial cells cultured in high glucose" provenance.
- _3 value "Summary of cDNAs identified by SSH as being down-regulated in mesangial cells cultured in high glucose" provenance.
- _3 value "Summary of cDNAs identified by SSH as being up-regulated in mesangial cells cultured in high glucose" provenance.
- _3 value "Summary of cDNAs identified by SSH as being up-regulated in mesangial cells cultured in high glucose" provenance.
- _3 value "Summary of cDNAs identified by SSH as being up-regulated in mesangial cells cultured in high glucose" provenance.
- _3 value "Summary of cDNAs identified by SSH as being up-regulated in mesangial cells cultured in high glucose" provenance.
- _2 value "induction of actin cytoskeleton regulatory gene expression by high glucose was attenuated by the inhibitor of reactive oxygen species generation, carbonyl cyanide m-chlorophenylhydrazone" provenance.
- _5 value "Bacterial lipoteichoic acid activates the platelet-activating factor receptor, which is G protein-coupled. This results in activation of a disintegrin and metalloproteinase (ADAM10), kuzbanian, cleavage of pro heparin-binding epidermal growth factor and activation of the epidermal growth factor receptor. " provenance.
- _4 value "Circulating leptin levels were almost 3 fold elevated in older (8-10 month old) but not in younger (3-month-old) female and male Il6-/- mice compared with their respective wild type mice." provenance.
- _3 value "During the differentiation of myoblasts to form myotubes, the expression patterns of the different actin isoforms change. The cytoplasmic actins, beta and gamma, are down-regulated and the muscle specific isoforms are up-regulated." provenance.
- _5 value "From mutations file" provenance.
- _5 value "Modified assertion" provenance.
- _3 value "insulin down-regulates IRS-1, IRS-2, and Shc gene expression in skeletal muscle in diabetic and nondiabetic monozygotic twins and control subjects to the same extent" provenance.
- _7 value "Suv39H1 bound the so-called 'core histone deacetylase complex', composed of HDAC1, HDAC2 and the Rb-associated proteins RbAp48 and RbAp46. Taken together, our results suggest that a complex containing both the Suv39H1 histone methyl transferase and histone deacetylases could be involved in heterochromatin silencing or transcriptional repression by Rb." provenance.
- _4 value "Proteomic methods have been used to monitor changes in protein synthesis in the first 4 h following stimulation of human lung fibroblasts with endothelin-1. Using pulsed [(35)S]methionine labeling, about 70 proteins with altered protein synthesis could be detected, and the 35 proteins showing the largest changes were identified by mass spectrometry." provenance.
- _4 value "Proteomic methods have been used to monitor changes in protein synthesis in the first 4 h following stimulation of human lung fibroblasts with endothelin-1. Using pulsed [(35)S]methionine labeling, about 70 proteins with altered protein synthesis could be detected, and the 35 proteins showing the largest changes were identified by mass spectrometry." provenance.
- _5 value "Proteomic methods have been used to monitor changes in protein synthesis in the first 4 h following stimulation of human lung fibroblasts with endothelin-1. Using pulsed [(35)S]methionine labeling, about 70 proteins with altered protein synthesis could be detected, and the 35 proteins showing the largest changes were identified by mass spectrometry." provenance.
- _5 value "Proteomic methods have been used to monitor changes in protein synthesis in the first 4 h following stimulation of human lung fibroblasts with endothelin-1. Using pulsed [(35)S]methionine labeling, about 70 proteins with altered protein synthesis could be detected, and the 35 proteins showing the largest changes were identified by mass spectrometry." provenance.
- _5 value "Proteomic methods have been used to monitor changes in protein synthesis in the first 4 h following stimulation of human lung fibroblasts with endothelin-1. Using pulsed [(35)S]methionine labeling, about 70 proteins with altered protein synthesis could be detected, and the 35 proteins showing the largest changes were identified by mass spectrometry." provenance.
- _5 value "Proteomic methods have been used to monitor changes in protein synthesis in the first 4 h following stimulation of human lung fibroblasts with endothelin-1. Using pulsed [(35)S]methionine labeling, about 70 proteins with altered protein synthesis could be detected, and the 35 proteins showing the largest changes were identified by mass spectrometry." provenance.
- _5 value "Proteomic methods have been used to monitor changes in protein synthesis in the first 4 h following stimulation of human lung fibroblasts with endothelin-1. Using pulsed [(35)S]methionine labeling, about 70 proteins with altered protein synthesis could be detected, and the 35 proteins showing the largest changes were identified by mass spectrometry." provenance.
- _4 value "Ariadne: Upon binding to its receptors, IL-18 induces activation of IL-1 receptor-associated kinase and TNF receptor-associated factor-6 (TRAF-6) and nuclear translocation of nuclear factor kappa-B (NFkappa-B) ( 13 , 14 ). [Regulation]" provenance.
- _4 value "In Jurkat cells Bid was cleaved upon activation of the Fas receptor with an anti-Fas antibody. The caspase-8 inhibitor benzyloxycarbonyl-Ile-Glu(OMe)-Thr-Asp(OMe)-CH(2)F (IETD) prevented the cleavage of Bid and the loss of viability." provenance.
- _6 value "After treatment with L739749 for 5 d (Fig. 2 F), vimentin and TGF expression was no longer detectable in the hollow structures obtained (Fig. 2 F, top). Two additional mesenchymal markers, CD68 (macrosialin) and calgranulin A, were also downregulated (unpublished data)." provenance.
- _8 value "Further work revealed that TGF-R signaling (Massague and Wotton, 2000) was required for EMT, invasion in vitro, and metastasis in vivo," provenance.
- _3 value "Ha-Ras cooperates with transforming growth factor beta (TGFbeta) to cause epithelial mesenchymal transition (EMT) characterized by spindle-like cell morphology, loss of epithelial markers, and induction of mesenchymal markers." provenance.