Matches in Nanopublications for { ?s ?p ?o <http://purl.org/np/RA6F1VxP7thp4zX-1raNBn2QGqYfK0d8XZO_-jUUWiqNE#assertion>. }
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- step type Step assertion.
- OBI_0002488 label "pipette" assertion.
- OBI_0000079 label "culture medium" assertion.
- _3 label "TE" assertion.
- _1 label "mycobacteria" assertion.
- _2 label "mycobacteria" assertion.
- step reagentUsed OBI_0000079 assertion.
- step reagentUsed _3 assertion.
- step description "Harvest mycobacteria by centrifuging 20-40 ml log phase culture (O.D.600 ≈ 0.8) at 2,000 x g for 10 min, room temperature. Carefully discard culture medium and add 10 ml TE (at room temperature) buffer to pellet. Mix gently with a pipette. Note: If isolating DNA from pathogenic mycobacteria such as M. tuberculosis, step 1 and 2 must be performed in a BSL-3 facility." assertion.
- step bioSampleUsed _2 assertion.
- step hasExperimentalInput _1 assertion.
- step labEquipmentUsed OBI_0002488 assertion.