Matches in Nanopublications for { ?s ?p ?o <http://purl.org/np/RAEdi_y8iLSbMtoWsgf1sBxHLPnZFDhYcBOF1e3xqonVA#assertion>. }
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- step type Step assertion.
- _1 label "extraction buffer" assertion.
- BAO_0010014 label "50 mL Falcon tube" assertion.
- PO_0025034 label "leaf tissues" assertion.
- step reagentUsed _1 assertion.
- step description "Take one gram of frozen finely powdered leaf tissues in a new 50 mL Falcon tube and mixed with the pre-heated extraction buffer (10 ml) for one sample. Breifly, vertex for 30sec to ensure leaf material is fully mixed with buffer. Fine grind is a key to obtaining high DNA quantity with lesser artifacts of resin." assertion.
- step bioSampleUsed PO_0025034 assertion.
- step hasExperimentalInput PO_0025034 assertion.
- step labEquipmentUsed BAO_0010014 assertion.