Matches in Nanopublications for { ?s ?p ?o <http://purl.org/np/RAKzzNvwKd7CCCX7I-7_BjKcZ7yyC9qoZiNar5Ly0RfIU#assertion>. }
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- step type Step assertion.
- OBI_0000918 label "DMEM" assertion.
- _1 label "cells" assertion.
- step reagentUsed OBI_0000918 assertion.
- step description "Wash cells (centrifuge at 100 x g for 10 min) with 10 ml of sterile DMEM (without FBS) twice. Note: Cold DMEM is not used routinely for washing lymphocytes from culture cavities while setting up cultures. Rather when monocytes bound tightly to plastic cavities are needed to be harvested pre-chilled DMEM can be used." assertion.
- step bioSampleUsed _1 assertion.