Matches in Nanopublications for { ?s ?p ?o <http://purl.org/np/RAYehDuYU12FcErOeUL91Sm9D92FV23Vh4Bn4bmtC7cYE#assertion>. }
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- step type Step assertion.
- _5 label "5 M NaCl " assertion.
- _4 label "RNAse A" assertion.
- _3 label "chloroform: isoamyl alcohol" assertion.
- _2 label "clear supernatant" assertion.
- _1 label "clear supernatant" assertion.
- BAO_0010014 label "tube" assertion.
- step reagentUsed _5 assertion.
- step reagentUsed _4 assertion.
- step reagentUsed _3 assertion.
- step description "After RNAse A treatment, add one volume of chloroform: isoamyl alcohol again and mix by inversions for 5 min. The tube is centrifuged for 10 min at 5000 × g at 4°C. Transfer the clear supernatant into new 2ml tube and add half volume of 5 M NaCl to the sample and mix gently by inversions" assertion.
- step hasExperimentalOutput _1 assertion.
- step bioSampleUsed _2 assertion.
- step labEquipmentUsed BAO_0010014 assertion.