Matches in Nanopublications for { ?s ?p ?o <http://purl.org/np/RAgJjjShkAgDJRhJ4KeEWjk2jxUkfUfuY5d-2UOUxv_NQ#assertion>. }
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- step type Step assertion.
- _1 label "PBS" assertion.
- OBI_0000918 label "Dulbecco's modified eagle medium" assertion.
- step reagentUsed _1 assertion.
- step reagentUsed OBI_0000918 assertion.
- step description "Wash (centrifuge in 100 x g for 10 min) twice with 10 ml of sterile PBS or sterile Dulbecco's modified eagle medium. The approximate yield of cells from 4 ml of blood varies between 107-108." assertion.