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- _6 label "Selventa" provenance.
- large_corpus.bel rights "Copyright (c) 2011-2012, Selventa. All rights reserved." provenance.
- _5 value "Next, we sought to analyse whether the decrease in FADD-FLICE association caused by PED overexpression affected FLICE protease activity. To this end, we incubated identical amounts of in vitro translated 35S-PARP, a death substrate cleaved by the active FLICE, with extracts from control or PED-overexpressing cells stimulated with IgMFAS. A major band, corresponding to the 26 kDa cleavage product of 35S-PARP was identified after incubation with extracts from IgMFAS-stimulated control cells (Figure 8). The intensity of the band was fivefold lower after PARP incubation with extracts from FAS-unstimulated cells. Most important, in this assay, PARP cleavage was threefold less effective in PED overexpressing than in control cells. No 26 kDa band was detectable in the absence of cell extract addition. Thus, cleavage of death substrates by active FLICE occurs less efficiently in cells overexpressing PED." provenance.
- _5 wasQuotedFrom 10442631 provenance.
- assertion hadPrimarySource 10442631 provenance.
- large_corpus.bel title "BEL Framework Large Corpus Document" provenance.
- large_corpus.bel description "Approximately 61,000 statements." provenance.
- assertion wasDerivedFrom large_corpus.bel provenance.
- assertion wasDerivedFrom _5 provenance.
- large_corpus.bel authoredBy _6 provenance.
- large_corpus.bel version "1.4" provenance.