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- _6 label "Selventa" provenance.
- large_corpus.bel rights "Copyright (c) 2011-2012, Selventa. All rights reserved." provenance.
- _5 value "Figure 5B shows that addition of either of the two anti-NFYA antibodies to the binding reactions supershifted a prominent complex (lanes 3 and 4) and the anti-NFYB antibody diminished binding significantly (lane 5). To test the functional effect of NFY binding to the +60/+64 region, we introduced point mutations abolishing the binding of NFY to its site (see Fig 5A and Fig 5B; lane 14) and incorporated them into a luciferase plasmid containing base pairs -391/+175 from the human CD34 promoter (constructs diagramed in Fig 5C). As shown in Fig 5D, mutating the NFY site (Y-M+ construct) resulted in a 5-fold decrease in luciferase activity in transiently transfected KG1a cells and a 4-fold decrease in RPMI-8402 cells, as compared with the wild-type construct (Y+M+ construct)." provenance.
- _5 wasQuotedFrom 10572091 provenance.
- assertion hadPrimarySource 10572091 provenance.
- large_corpus.bel title "BEL Framework Large Corpus Document" provenance.
- large_corpus.bel description "Approximately 61,000 statements." provenance.
- assertion wasDerivedFrom large_corpus.bel provenance.
- assertion wasDerivedFrom _5 provenance.
- large_corpus.bel authoredBy _6 provenance.
- large_corpus.bel version "1.4" provenance.