Matches in Nanopublications for { ?s ?p ?o <http://www.tkuhn.ch/bel2nanopub/RA8hglCFNv_9ntjaGHJosMwPIMOZl1VFh1zAje9EhDpQM#provenance>. }
Showing items 1 to 11 of
11
with 100 items per page.
- _4 label "Selventa" provenance.
- large_corpus.bel rights "Copyright (c) 2011-2012, Selventa. All rights reserved." provenance.
- _3 value "BACKGROUND: It appears that consistent changes in the levels of activity of a small cohort of genes (probably mammalian cells during ageing. Identification of such genes could provide valuable insights into the ageing process. OBJECTIVE: We have studied age-related changes in gene expression profiles in the rat liver. One of the genes which exhibited clear and consistent increases in expression is characterised. METHODS: Analysis of the gene expression profile was carried out using a combination of an optimised form of differential display and single-strand conformational polymorphism (SSCP) gel analysis. Gene expression levels were quantified by Northern blot analysis. The gene's identity was determined by comparing its nucleotide sequence to DNA databases. RESULTS: During this investigation we observed one gene which exhibited an increase in expression in livers from young adult (6 months) to old adult (24 months) rats. The differential expression of this gene was confirmed by SSCP gel analysis and Northern blotting. Densitometry of the latter indicated that expression increased by 165% with age. Characterisation of the isolated polymerase chain reaction fragment demonstrated it to code for the ribosomal protein S25 (RPS25). CONCLUSIONS: This increase in RPS25 expression is further evidence that the composition of ribosomes may alter with age and as a result could have significant effects on protein synthesis." provenance.
- _3 wasQuotedFrom 12393952 provenance.
- assertion hadPrimarySource 12393952 provenance.
- large_corpus.bel title "BEL Framework Large Corpus Document" provenance.
- large_corpus.bel description "Approximately 61,000 statements." provenance.
- assertion wasDerivedFrom _3 provenance.
- assertion wasDerivedFrom large_corpus.bel provenance.
- large_corpus.bel authoredBy _4 provenance.
- large_corpus.bel version "1.4" provenance.