Matches in Nanopublications for { ?s <http://www.w3.org/ns/prov#value> ?o ?g. }
- _6 value "Modified assertion" provenance.
- _5 value "Mercader et al. (1999) described the role of homeobox genes Meis1, Meis2, and Pbx1 (176310) in the development of mouse, chicken, and Drosophila limbs. Mercader et al. (1999) found that Meis1 and Meis2 expression is restricted to the proximal domain, coincident with the previously reported domain in which Pbx1 is localized to the nucleus. Meis1 regulates Pbx1 activity by promoting nuclear import of the Pbx1 protein. Mercader et al. (1999) also demonstrated that ectopic expression of Meis1 in chicken disrupts distal limb development and induces distal-to-proximal transformations. Mercader et al. (1999) concluded that the restriction of Meis1 to proximal regions of the vertebrate limb is essential to specify cell fates and differentiation patterns along the proximodistal axis of the limb." provenance.
- _5 value "a dominant-negative mutant of nuclear factor-kappaB-inducing kinase also promoted TNFalpha- and Fas-mediated apoptosis. Expression of either crmA or a dominant-negative mutant of the Fas-associated death domain protein prevented TNFalpha- and Fas-mediated apoptosis. In addition, the caspase inhibitors, DEVD-cho and IETD-fmk, inhibited TNFalpha- and Fas-mediated apoptosis. In Ad5IkappaB-infected hepatocytes, caspases-3 and -8 were activated within 2 h after treatment with anti-Fas antibody or within 6 h after TNFalpha treatment" provenance.
- _4 value "1. The isoflavone genistein may either stimulate or inhibit cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channels. To investigate how genistein inhibits CFTR, we studied CFTR Cl- channels in excised inside-out membrane patches from cells expressing wild-type human CFTR. 2. Addition of genistein (100 microM) to the intracellular solution caused a small decrease in single-channel current amplitude (i), but a large reduction in open probability (Po). 3. Single-channel analysis of channel block suggested that genistein (100 microM) may inhibit CFTR by two mechanisms: first, it may slow the rate of channel opening and second, it may block open channels. 4. Acidification of the intracellular solution relieved channel block, suggesting that the anionic form of genistein may inhibit CFTR. 5. Genistein inhibition of CFTR Cl- currents was weakly voltage dependent and unaffected by changes in the extracellular Cl- concentration. 6. Channel block was relieved by pyrophosphate (5 mM) and ATP (5 mM), two agents that interact with the nucleotide-binding domains (NBDs) of CFTR to greatly stimulate channel activity. 7. ATP (5 mM) prevented the genistein-induced decrease in Po, but was without effect on the genistein-induced decrease in i. 8. The genistein-induced decrease in i was voltage dependent, whereas the genistein-induced decrease in Po was voltage independent. 9. The data suggest that genistein may inhibit CFTR by two mechanisms. First, it may interact with NBD1 to potently inhibit channel opening. Second, it may bind within the CFTR pore to weakly block Cl- permeation." provenance.
- _3 value "Overexpression of 14-3-3 sigma can also inhibit cell proliferation and prevent anchorage-independent growth of these cell lines." provenance.
- _3 value "Indeed, the secretion of TGF-beta proteins into the culture media was increased after stretch. Stretch also stimulated mRNA expression of the ECM components, type I and type IV collagen, and fibronectin, which was largely inhibited by addition of neutralizing antibody against TGF-beta." provenance.
- _5 value "Expression of Bam32 in B cells leads to a dose-dependent inhibition of BCR-induced activation of nuclear factor of activated T cells (NF-AT), which is blocked by deletion of the PH domain or mutation of the PI(3,4,5)P(3)-binding motif. Thus, Bam32 represents a novel B cell-associated adaptor that regulates BCR signaling downstream of PI3K." provenance.
- _3 value "It is thought that neutrophils are attracted to the skin by an array of chemotactic factors (Camp 1989;Camp et al. 1990). These include a number of neutrophil-activating polypeptide species such as interleukin-8 (IL-8), gro/MGSA (growth-related gene/melanoma growth stimulatory activity), or the complement split product C5ades arg (Schr�der et al. 1987,1988a,1998b,van Damme et al. 1988;Baggiolini et al. 1989;Gillitzer et al. 1996). " provenance.
- _5 value "Putative binding sites for the erythroid-specific nuclear factors GATA-1, NF-E2, and EKLF were identified within the first 300bp region of the mouse ALAS2 5'-flanking region" provenance.
- _7 value "Treatment with TNFalpha at concentrations of 0.1-10 ng/ml resulted in a dose dependent increase in 11beta-HSD1 reductase activity from 1.5 to 10-fold. Insulin (0.1-100 nM) had no effect under basal conditions, but inhibited the stimulatory effects of TNFalpha (5 ng/ml) on 11beta-HSD1 reductase activity in a dose dependent fashion (8-66%) inhibition)." provenance.
- _4 value "The expression of GLUT1 mRNA increased after 4 h of stimulation with IGF-I and decreased after 24 h. Blocking PI3K activity by inhibitor wortmannin abolished the effect of IGF-I on Glut1 mRNA expression in NIH-IGFR cells suggesting the involvement PI3K." provenance.
- _6 value "Modified assertion" provenance.
- _6 value "Modified assertion" provenance.
- _3 value "PMCA1 was repressed approximately 33% after a high, but not a low dose of the GC, corticosterone (B), suggesting glucocorticoid (but not mineralocorticoid) receptor-mediated repression." provenance.
- _5 value "Phosphorylation of two closely spaced serine residues in this domain, at positions 176 and 180 in IKK? and positions 177 and 181 in IKK?, has been shown to be important for IKK kinase activity (22). " provenance.
- _6 value "Different agents activated the 5-LO kinase activities, including stimuli for cellular leukotriene biosynthesis (A23187, thapsigargin, N-formyl-leucyl-phenylalanine), compounds that up-regulate the capacity for leukotriene biosynthesis (phorbol 12-myristate 13-acetate, tumor necrosis factor alpha, granulocyte/macrophage colony-stimulating factor), and well known p38 stimuli as sodium arsenite and sorbitol. For all stimuli, 5-LO kinase activation was counteracted by SB203580 (3 microM or less), an inhibitor of p38 kinase" provenance.
- _4 value "Using a transient transfection approach in a mature B cell line, 2PK3, we demonstrate that RGS1 and RGS3s are effective inhibitors of chemotaxis toward the lymphoid tissue chemokines stromal cell-derived factor-1, B lymphocyte chemoattractant, and EBV-induced molecule 1 ligand chemokine, whereas RGS2 has a minimal effect on migration to these chemokines." provenance.
- _3 value "ADAMTS-1 and cathepsin L are transcriptional targets of PR action. ADAMTS-1 is induced after LH stimulation in granulosa cells of preovulatory follicles and depends on PR." provenance.
- _4 value "Our results indicate that MEF2C can directly or indirectly activate the expression of MASH-1, leading to neurogenesis." provenance.
- _4 value "PA28 is an interferon-gamma inducible modulator of proteasome function composed of two subunits, i.e. PA28alpha and PA28beta." provenance.
- _3 value "However, GM-CSF mRNA was induced by PMA, but with a delayed time course in comparison with IL-1b (Figure 7A)." provenance.
- _3 value "Supervillin, a membrane-associated, F-actin-binding protein, was identified as one of the testosterone downregulated genes in frontal DPCs." provenance.
- _3 value "Figure 2 in the actual paper." provenance.
- _3 value "Figure 2 in the actual paper." provenance.
- _5 value "We report here that the serine proteinase inhibitor (serpin) PI-9 accounts for the endogenous caspase-1 inhibitory activity in human SMCs and prevents processing of the enzyme's natural substrates, IL-1beta and IL-18 precursor." provenance.
- _8 value "We report here that the serine proteinase inhibitor (serpin) PI-9 accounts for the endogenous caspase-1 inhibitory activity in human SMCs and prevents processing of the enzyme's natural substrates, IL-1beta and IL-18 precursor." provenance.
- _4 value "In in vitro cell culture study, 5-day continuous suppression of H-ras expression by antisense H-ras oligodeoxynucleotide resulted in significant inhibition of the proliferation of LCI-D20 cells (t = 31.529, P < 0.01). " provenance.
- _3 value "Using two structurally unrelated PI3K inhibitors, wortmanin and LY294002, both tyrosine phosphorylation of Cx43 and activation of ERK stimulated by PDGF were largely blocked." provenance.
- _3 value "Using two structurally unrelated PI3K inhibitors, wortmanin and LY294002, both tyrosine phosphorylation of Cx43 and activation of ERK stimulated by PDGF were largely blocked." provenance.
- _4 value "At the posttranslational level, alpha-tocopherol inhibits protein kinase C, 5-lipoxygenase and phospholipase A2 and activates protein phosphatase 2A and diacylglycerol kinase" provenance.
- _3 value "Quercetin (inhibitor of PLA(2) and lipoxygenase) inhibited only the OS-induced but not the HS-induced expression of Hsp68." provenance.
- _2 value "Fig. 3" provenance.
- _2 value "Insulin reduces the blood glucose level by enhancing glucose uptake, mainly in the muscle and liver, thereby promoting glucose oxidation and glycogen synthesis." provenance.
- _2 value "JNK is required for UV-induced apoptosis in primary murine embryonic fibroblasts" provenance.
- _6 value "These results demonstrate an additional and novel function for caspase-1 in which Pro-C1 acts to enhance Fas-mediated apoptosis, most probably through facilitation of the activation of caspase-8" provenance.
- _5 value "Modified assertion" provenance.
- _3 value "# Ariadne: Therefore, the present study demonstrates that PGE 2 has the ability to stimulate AP-1-mediated expression of CD14 in mouse macrophages via cAMP-dependent protein kinase A. [Expression] # Ariadne: PGE 2 -stimulated expression of CD14 was inhibited by antisense c- fos and c- jun oligonucleotides, but not by their sense oligonucleotides. [Expression]" provenance.
- _6 value "# Ariadne: Therefore, the present study demonstrates that PGE 2 has the ability to stimulate AP-1-mediated expression of CD14 in mouse macrophages via cAMP-dependent protein kinase A. [Expression] # Ariadne: PGE 2 -stimulated expression of CD14 was inhibited by antisense c- fos and c- jun oligonucleotides, but not by their sense oligonucleotides. [Expression]" provenance.
- _3 value "PGE2 also stimulated activation of AP-1, a heterodimer of c-Jun and c-Fos" provenance.
- _6 value "Protein kinase A inhibitor, N-[2-(p-bromocinnamylamino)ethyl]-5- isoquinolinesulfonamide (H-89), but not protein kinase C inhibitor 3-(1-[3- (dimethylamino)propyl]-1H- indol-3-yl)-4-(1H-indol-3-yl)-1H-py rrole-2,5-dione (GF109203X), abolished the stimulated expression of CD14." provenance.
- _3 value "we investigated the effect of PG on CD14 expression in mouse macrophages. PGE1, PGE2, and PGA1 among the PGs tested strongly stimulated the expression of the CD14 gene in the cells." provenance.
- _5 value "The biological activity of u-PA, t-PA, as well as that of limiting concentration of FCS (1%), was mediated by PTK and PKC." provenance.
- _4 value "Factor XII deficiency has been postulated to be a risk factor for thrombosis suggesting that factor XII is an antithrombotic protein. The biochemical mechanism leading to this clinical observation is unknown. We have previously reported high molecular weight kininogen (HK) inhibition of thrombin-induced platelet aggregation by binding to the platelet glycoprotein (GP) Ib-IX-V complex. Although factor XII will bind to the intact platelet through GP Ibalpha (glycocalicin) without activation, we now report that factor XIIa (0. 37 microm), but not factor XII zymogen, is required for the inhibition of thrombin-induced platelet aggregation. Factor XIIa had no significant effect on SFLLRN-induced platelet aggregation. Moreover, an antibody to the thrombin site on protease-activated receptor-1 failed to block factor XII binding to platelets. Inhibition of thrombin-induced platelet aggregation was demonstrated with factor XIIa but not with factor XII zymogen or factor XIIf, indicating that the conformational exposure of the heavy chain following proteolytic activation is required for inhibition. However, inactivation of the catalytic activity of factor XIIa did not affect the inhibition of thrombin-induced platelet aggregation. Factor XII showed displacement of biotin-labeled HK (30 nm) binding to gel-filtered platelets and, at concentrations of 50 nm, was able to block 50% of the HK binding, suggesting involvement of the GP Ib complex. Antibodies to GP Ib and GP IX, which inhibited HK binding to platelets, did not block factor XII binding. However, using a biosensor, which monitors protein-protein interactions, both HK and factor XII bind to GP Ibalpha. Factor XII may serve to regulate thrombin binding to the GP Ib receptor by co-localizing with HK, to control the extent of platelet aggregation in vivo." provenance.
- _5 value "# Ariadne: Ubiquitin- conjugated keratin 8 fragments appear to accumulate in colorectal carcinomas ( 16 ). [Binding]" provenance.
- _4 value "its immunoreactivity with RS-11 is up-regulated by malignant transformation or stimulation with either epidermal growth factor or transforming growth factor alpha." provenance.
- _5 value "PACAP induced expression of GLAST via activation of PKA-dependent signaling pathways only, but it affected the expression of GLT-1 via both PKA and PKC-dependent signaling pathways." provenance.
- _4 value "In 7-d-old rat pups, we found that (1) postnatal handling increased adenylyl cyclase activity and hippocampal cAMP levels," provenance.
- _6 value "In this study, we demonstrate that PI3K-C2alpha and PI3K-C2beta represent two downstream targets of the activated epidermal growth factor (EGF) receptor in human carcinoma-derived A431 cells. " provenance.
- _5 value "Activation of p38 also enhances the transcriptional activities of myocyte enhancer binding factor 2A (MEF2A) and MEF2C by direct phosphorylation" provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _3 value "Interleukin (IL)-10 suppresses macrophage activation and down-regulates proinflammatory cytokine production" provenance.
- _3 value "mRNA expression of 2 PTPases (PTPL1 and PTP1B) was specifically upregulated by proliferating and migrating smooth muscle cells (SMCs) in characteristic temporal and regional patterns in response to vessel damage." provenance.
- _5 value "PTPN1 binding is required for the physiological effects of PTPN1 on IR signaling transduction (dephosphorylation)" provenance.
- _4 value "This phosphorylation event could only be inhibited by K-252a and stauroporin." provenance.
- _3 value "The skeletal muscle sarco(endo)plasmic reticulum calcium ATPase (SERCA1) gene is transactivated as early as 2 days after the removal of weight-bearing (Peters, D. G., Mitchell-Felton, H., and Kandarian, S. C. (1999) Am. J. Physiol. 276, C1218-C1225), but the transcriptional mechanisms are elusive." provenance.
- _3 value "Table 1. Transcripts differentially regulated in high vs. low glucose" provenance.
- _3 value "Table 1. Transcripts differentially regulated in high vs. low glucose" provenance.
- _3 value "Table 1. Transcripts differentially regulated in high vs. low glucose" provenance.
- _3 value "Table 1. Transcripts differentially regulated in high vs. low glucose" provenance.
- _3 value "Table 1. Transcripts differentially regulated in high vs. low glucose" provenance.
- _3 value "Table 1. Transcripts differentially regulated in high vs. low glucose" provenance.
- _3 value "Table 1. Transcripts differentially regulated in high vs. low glucose" provenance.
- _3 value "Table 1. Transcripts differentially regulated in high vs. low glucose" provenance.
- _3 value "Table 1. Transcripts differentially regulated in high vs. low glucose" provenance.
- _3 value "Table 1. Transcripts differentially regulated in high vs. low glucose" provenance.
- _3 value "Table 1. Transcripts differentially regulated in high vs. low glucose" provenance.
- _4 value "TSC-36 (TGF-beta1-stimulated clone 36) is a TGF-beta1 inducible gene whose product is an extracellular glycoprotein that contains a single follistatin module. %TSC-36 is highly expressed in the lung, but its physiological function is unknown." provenance.
- _3 value "RT-PCR experiments demonstrated an increased expression of clusterin, C1qB, and C4 mRNA in the ischemic cortex, with a peak level at 3 days after MCA occlusion." provenance.
- _3 value "RT-PCR experiments demonstrated an increased expression of clusterin, C1qB, and C4 mRNA in the ischemic cortex, with a peak level at 3 days after MCA occlusion." provenance.
- _5 value "title := \"The steroid receptor coactivator, GRIP-1, is necessary for MEF-2C-dependent gene expression and skeletal muscle differentiation.\"" provenance.
- _3 value "Differentiation of U937 and HPM cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) enhanced the H1 receptor expression and rather suppressed the H2 receptor, resulting in up-regulation of the histamine-induced expression and secretion of TNF-alpha" provenance.
- _3 value "histamine suppresses gene expression and synthesis of tumor necrosis factor alpha (TNF-alpha) induced by lipopolysaccharide (LPS)" provenance.
- _3 value "# Ariadne: On the basis of our current results and those of others (Legay et al., 1995 ; see also Fontaine and Changeux, 1989 ; Tsay and Schmidt, 1989 ; Piette et al., 1993 ), it appears that during synaptogenesis and maturation of the neuromuscular junction, the activity-linked regulation of the AChE gene involves transcriptional and posttranscriptional regulatory mechanisms and that, in mature muscle, regulation of this synaptic protein is achieved primarily via posttranscriptional events. [Regulation] The molecular mechanisms underlying the activity-linked plasticity of acetylcholinesterase (AChE) mRNA levels in mammalian skeletal muscle have yet to be established. Here, we demonstrate that denervation of adult muscle induces a dramatic (up to 90%) and rapid (within 24 h) decrease in the abundance of AChE mRNAs." provenance.
- _4 value "Modified assertion" provenance.
- _6 value "Expression of active PTPH1 also reduced receptor-induced activation of Erk2 MAP kinase, its upstream activator, Mek, and the Jnk kinases." provenance.
- _5 value "Second, using confocal microscopy, we show that insulin induces translocation of SOCS-3 from an intracellular compartment to the cell membrane, leading to colocalization of SOCS-3 with the insulin receptor. This colocalization is dependent upon phosphorylation of insulin receptor tyrosine 960." provenance.
- _5 value "Using transfection of COS-7 cells, we show that insulin induction of SOCS-3 is enhanced upon Stat5B expression. Moreover, Stat5B from insulin-stimulated cells binds directly to a Stat element present in the SOCS-3 promoter." provenance.
- _4 value "Using transfection of COS-7 cells, we show that insulin induction of SOCS-3 is enhanced upon Stat5B expression. Moreover, Stat5B from insulin-stimulated cells binds directly to a Stat element present in the SOCS-3 promoter." provenance.
- _4 value "Using transfection of COS-7 cells, we show that insulin induction of SOCS-3 is enhanced upon Stat5B expression. Moreover, Stat5B from insulin-stimulated cells binds directly to a Stat element present in the SOCS-3 promoter." provenance.
- _5 value "When overexpressed in C2C12 myoblasts, wild type SOX15, but not a C-terminal truncated form or the related protein SOX11, specifically inhibited activation of muscle-specific genes and expression of the basic helix-loop-helix myogenic factors myogenin and MyoD" provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _5 value "Modified assertion" provenance.
- _8 value "We found that although both EGF and FGF induce activation of mitogen-activated protein kinase (MAPK), LAR only inhibits FGF-induced MAPK activation. " provenance.
- _5 value "Loss of alpha -Adducin from Adherens Junctions Is Associated with Increased Phosphorylation of Ser726-- Phosphorylation of alpha - and gamma -adducin at Ser726 and Ser660 precedes loss of both alpha - and gamma -adducin from adherens junctions after treatment of RPTE cells with the phorbol ester phorbol 12,13-dibutyrate (41, 58) [activates PKC]." provenance.
- _7 value "Introduction of an additional mutation (Y694F) to STAT5A-N642H, which disrupted critical tyrosine 694 required for dimerization of STAT5, abolished all the activities manifested by the mutant STAT5A-N642H, which indicates that dimerization is required for the activity of STAT5A-N642H as was the case for the wild-type STAT5A. T" provenance.
- _6 value "We first observed that in the absence of Jak3, both Lck and Syk had the capacity to phosphorylate Stat3 and Stat5a." provenance.
- _6 value "We first observed that in the absence of Jak3, both Lck and Syk had the capacity to phosphorylate Stat3 and Stat5a." provenance.
- _5 value "insulin significantly increased MAP kinase [isoforms p42(MAPK) and p44(MAPK) (ERK1 and ERK2)] phosphorylation and activity, and the activity of its upstream activator MEK1. Insulin also increased the activity of the MAP kinase downstream substrate, the p90 ribosomal S6 kinase 2 (RSK2) almost twofold" provenance.
- _4 value "The ligand for the receptor tyrosine kinase fms-like tyrosine kinase 3 (flt3), also referred to as fetal liver kinase-2 (flk-2), has an important role in hematopoiesis. The flt3 ligand (flt3L) is a growth factor for hematopoietic progenitors and induces hematopoietic progenitor and stem cell mobilization in vivo." provenance.
- _5 value "the ability of epidermal growth factor to inhibit PDE4D3 through ERK2 action inherent inhibitory effect that ERK2 phosphorylation exerts on the PDE4D catalytic unit" provenance.
- _5 value "Moreover, Rac1 and Cdc42Hs extinction in normal cells activates endogenous p53." provenance.
- _2 value "Cells treated with hydrogen peroxide, EGTA or BAPTA-AM, to remove calcium did not stimulate conversion of p35 to p25 following hydrogen peroxide treatment." provenance.
- _3 value "Oral treatment of animals with all-trans-retinoic acid for one and two days induced a significant decrease of FGF-BP protein in tissues from stomach, eye and lung suggesting that regulation of FGF-BP can be one effector mechanism through which retinoids affect normal and pathological processes." provenance.
- _3 value "Oral treatment of animals with all-trans-retinoic acid for one and two days induced a significant decrease of FGF-BP protein in tissues from stomach, eye and lung suggesting that regulation of FGF-BP can be one effector mechanism through which retinoids affect normal and pathological processes." provenance.
- _5 value "Inhibition of MEK reduced the activated and basal expression of claudin-2 messenger RNA and protein expression" provenance.
- _4 value "L-17 induced the development of a paracellular barrier of T84 cell monolayers. Inhibition of ERK activation with the MEK inhibitor PD98059 blocked IL-17 as well as basal development of tight junctions in T84 cells. IL-17 induced formation of tight junctions correlated with up-regulation of claudin-1 and claudin-2 gene transcription. Inhibition of MEK reduced the activated and basal expression of claudin-2 messenger RNA and protein expression. Functional MEK was required for the expression and membrane association of claudin-2 but not claudin-1 in T84 cells. CONCLUSIONS: MEK activity is required for claudin-mediated formation of tight junctions. IL-17 is able to regulate the intestinal barrier through the ERK MAPK pathway" provenance.
- _3 value "Modified assertion" provenance.
- _6 value "We then confirmed by flow cytometry that the observed increases in message levels were reflected in increased cell surface protein levels for ICAM-1 and MHC class I in RARbeta2 transfectants" provenance.
- _6 value "Histamine (10(-4) M) also led to an approximately 4.5-fold increase in Jun NH(2)-terminal kinase activity in a PKC-, PKA-, and MAP kinase-independent but rapamycin-sensitive manner" provenance.
- _4 value "activation of the human H(2) receptor (hH(2)R) leads to an increase in c-fos transcription and cell proliferation" provenance.