Matches in Nanopublications for { <http://purl.org/np/RAsJyZ5Znc54-tK37PmFveFPCA0USROZ7tO4XVMsraHW4#step> ?p ?o ?g. }
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- step type Step assertion.
- step reagentUsed OBI_0000918 assertion.
- step description "Wash cells (centrifuge at 100 x g for 10 min) with 10 ml of sterile DMEM (without FBS) twice. Note: Cold DMEM is not used routinely for washing lymphocytes from culture cavities while setting up cultures. Rather when monocytes bound tightly to plastic cavities are needed to be harvested pre-chilled DMEM can be used. " assertion.
- step precedes step assertion.
- step isStepOfPlan plan assertion.
- step hasExperimentalOutput _1 assertion.
- step bioSampleUsed _2 assertion.