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- _4 value "he microarray data set was validated by performing quantitative polymerase chain reaction (qPCR) on three independent pairs of subpallia from E14.5 mutant (Arx?/Y; Pou3f4-Cre+) and control (Arx+/Y; Pou3f4Cre+) embryos. A subset of 10 genes identified as differentially expressed, along with three genes that did not change significantly, were selected for testing. All 10 genes designated as differentially expressed by microarray analysis changed at least 2-fold by qPCR, whereas the three control genes that failed to change on the microarray also showed no difference in expression by qPCR (Fig. 2D). Log2 expression observed by microarray analysis was found to positively and significantly correlate to the log2 expression observed by qPCR (Pearson r2 = 0.7031, P < 0.001) (Fig. 2E)." provenance.
- _4 wasQuotedFrom 18799476 provenance.