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- _5 value "we transiently transfected the wild-type and CD40 cytoplasmic domain mutants with a plasmid containing the NF-kappaB response element upstream of a luciferase gene (pNF-kappaB-luc) and 2 ug of a Renilla luciferase (pHRL-luc) plasmid to normalize for transfection efficiency. The cells were subsequently stimulated via CD40 using CHO-CD154 for 16 h. Stimulation of both the wild-type and hCD 32 mutant resulted in a 3-fold induction of NF-kappaB luciferase activity above background, whereas stimulation of the hCD40EEAA mutant did not result in detectable stimulation of NF-kappaB reporter activity (Fig. 9A). Thus, optimal NF-kappaB activation requires an intact TRAF6 binding domain of CD40." provenance.
- _5 wasQuotedFrom 15634933 provenance.