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- _4 value "100 representative genes with -fold change values greater than or equal to 2 that were dependent on NF-kappa B for maintaining their relative expression levels were selected from a primary WT (+TNFalpha ) versus Ikappa Balpha SR (+TNFalpha ) screen. Genes were grouped in categories based upon their physiological functions or properties. Gene accession numbers are in the far left column adjacent to their names and descriptions. In the first data column, -fold changes of genes identified in two independent primary microarray screens of WT MEFs compared with WT. MEFs constitutively expressing the Ikappa Balpha (SS32/36AA) superrepressor are provided. In data columns 2-4, the dependences of each of these genes on IKKalpha , NEMO/IKKgamma , and IKKbeta were determined in six independent microarray screens wherein WT MEFs were compared with mutant MEFs null for the individual IKK subunits. -Fold change values from duplicate screenings were listed together. Dependence on one or more signalsome subunits was stringently evaluated by adhering to two criteria: wild type MEF absolute calls of \"present\" (P) and \"increase\" (I) average difference calls. Each screen was performed with cells stimulated for 2 h with 10 ng/ml human TNFalpha . Genes that were dependent on basal NF-kappa B for their expression were identified by performing independent microarray screens in the absence of TNFalpha stimulation. Redundancy hits (corresponding to different oligonucleotide regions of the same gene) are noted in the gene description column. NC denotes a \"no change\" average difference call, indicating no significant dependence on that IKK subunit. Results of exposure to TNFalpha are presented in the indicated column as follows: - (no significant effect), +/- (~2-5-fold stimulation), and + (>10-fold stimulated). Two independent screens were conducted in all cases with similar results." provenance.
- _4 wasQuotedFrom 12221085 provenance.