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- _3 value "Spontaneously immortalized wild type mouse fibroblasts were mock-irradiated or irradiated with x-rays and harvested after 30 min, and H2AX phosphorylation was analyzed by Western blotting of SDS extracts with anti-?-H2AX antibody. We observed significant phosphorylation of histone H2AX in response to ionizing radiation (Fig.1 b). The observed phosphorylation was specific to serine 139. Significant phosphorylation of H2AX was also observed after treatment of cells with the DSB-inducing agents neocarzinostatin, bleomycin, and etoposide. " provenance.
- _3 wasQuotedFrom 11571274 provenance.