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- _3 value "Human microvascular endothelial cells from lung (HMVEC-Lung, HMVEC-L) and cardiac (HMVEC-Cardiac, HMVEC-C) cells were cultured and exposed to hypoxia (1% O2, 5% CO2, and 94% N2) on 6 separate occasions, and total RNA was extracted. Experimental samples (n = 3) were formed by randomly pooling RNA from 2 independent experiments. This process was repeated at each of 4 experimental time points yielding 12 separate arrays for lung and cardiac experiments. Affymetrix GeneChip Human Genome U133A chips, which allow the simultaneous analysis of 22,283 gene transcripts, were used. * Genstruct R-pipeline analysis of GSE11341 conducted Nov 2010 by DD. Cutoffs for curation applied by AV were FC>1.5 and adj. p<.01. The 48h treatment timepoint was selected for curation because, as the paper reports, \"An incremental increase in the expression of 2 well-characterized hypoxia-responsive marker genes, namely VEGF-A and solute carrier family 2 member 1 [SLC2A1 (previously called GLUT1)], was measured by RT-PCR.\"" provenance.
- _3 wasQuotedFrom 18469115 provenance.