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- _5 value "VEGF induced tyrosine phosphorylation of the regulatory p85 subunit of PI 3-kinase, evidenced by immunoblotting of anti-p85 immunoprecipitates with an anti-pY mAb (Fig. 5b). Moreover, immunoblotting of anti-pY immunoprecipitates with specific p85 mAb showed an increased association of p85 with tyrosine-phosphorylated proteins, which was maximal after 5 min of VEGF treatment (Fig. 5c). To determine whether VEGF-induced p85 phosphorylation regulates p110, the catalytic counterpart of PI 3-kinase, we performed in vitrokinase assays using PI 4,5-bisphosphate as a substrate. As shown in Fig. 5d, VEGF induced peak activation (6-fold) of PI 3-kinase activity at 5 min." provenance.
- _5 wasQuotedFrom 11751905 provenance.