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- _5 value "The POU IV family members are the closest mammalian homologs to the nematode Unc-86. This, together with their restricted expression in the developing and adult nervous system, has led to the idea that they may play a role in regulating gene expression in neuronal cells. SNAP-25 is a presynaptic nerve terminal protein, and its promoter has been shown to be activated by POU4F3 only in neuron-derived cell lines such as PC12 and ND7. This activation depends on a neuron-specific activation domain in the amino terminus of POU4F3 (30). PC12 cells transfected with the pHM6 vector, pHM6-wt (wild-type POU4F3 cDNA), or pHM6-mut (del8 POU4F3 cDNA) constructs were examined for their ability to activate the SNAP-25 promoter connected to a luciferase reporter gene. The luciferase values were normalized for �-galactosidase levels. We found that the mutant product had only ca. 30% activity compared to the wild-type product [(1.3 � 0.07)-fold versus (2.02 � 0.06)-fold] " provenance.
- _5 wasQuotedFrom 14585957 provenance.