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- _4 value "In order to determine the functionality of the TNF-a released in cell supernatants in terms of NF-kB inducibility, we treated ACH-2 cells with equivalent TNF-a concentrations to those released in supernatants after treatment with H2O2 or HOCl, i.e. 100 and 33 pg/ml respectively (or 16.6 and 5 units/ml). Nuclear extracts were prepared 30, 60 and 120 min after induction and analysed by EMSA. As shown in Figure 7(A), a rather important specific NF-kB induction was detectable in the nucleus of ACH-2 cells treated under these conditions. " provenance.
- _4 wasQuotedFrom 9032466 provenance.