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- _5 value "Western blot analysis was performed on protein extracts isolated from HPECs overexpressing Cdc37 or the vector only (Fig. 1C) ? .Raf-1 steady-state levels were unchanged. However, Raf-1 activity, measured by the abundance of phosphorylated S217/221 MEK1/2, a direct target of Raf-1 (30) , was consistently elevated in Cdc37-overexpressing cells, whereas total MEK1/2 levels were unchanged. Increases in Cdk4 levels and markedly reduced p16 expression were also consistently observed in Cdc37-overexpressing HPECs. " provenance.
- _5 wasQuotedFrom 12907640 provenance.