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- _3 value "Regarding the molecular mechanisms by which insulin/IGF-I elicit the positive effects on adipogenic differentiation, the IRS/PI3K signalling pathway plays a major role. Effective inhibitors of PI3K may help to define the role of this enzyme and its products in cells. In this regard, the use of two unrelated chemical inhibitors of PI3K enzymatic activity, wortmannin (a cell-permeable fungal metabolite that blocks the catalytic activity of the enzyme by interfering with p85-p110 interaction without affecting upstream signalling events) (Okada et al. 1994) and LY294002 (that acts on the ATP-binding site of the enzyme) (Vlahos et al. 1994), has allowed us to define the contribution of this pathway in inducing the expression of various metabolic genes. Experiments performed in our laboratory have shown that the expression of FAS and malic enzyme induced by IGF-I or insulin was completely prevented by treatment of primary brown adipocytes with either wortmannin or LY294002." provenance.
- _3 wasQuotedFrom 15777261 provenance.