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- _3 value "Microarray analysis of the transcriptome of H1975 cells treated with BMS-690514 or CDDP. H1975 cells were treated with either 5 �mol/L (1 �mol/L) BMS-690514 and 50 �mol/L (25 �mol/L) CDDP for 24 h (48 h) and subjected to RNA isolation. Total RNA was then retrotranscribed into double-stranded cDNA followed by T7 RNA polymerase?mediated linear amplification, labeling, and hybridization to a human whole genome 44k oligonucleotide array. Samples from treated cultures were compared with cRNAs obtained through the same procedure from untreated cells. From Supplementary Table S1, which contains a complete list of genes that are transcriptionally modulated by BMS-690514 by a factor higher than 2. n.s., nonstatistically significant P value (>10?5). H1975 cells have a secondary somatic EGFR mutation T790M which confers resistance to erlotinib. BMS-690514, a novel panHER/VEGFR inhibitor described here, exerted antiproliferative and pro-apoptotic effects on NSCLC cell lines, with prominent efficacy on H1975 cells expressing the T790M mutation.(I included only 24h time points-JSP) " provenance.
- _3 wasQuotedFrom 17616683 provenance.