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- _4 value "Hyperosmotic stress (0�5 M sorbitol) in combination with protein Ser}Thr phosphatase inhibition (0�2 lM okadaic acid) induced a prolonged activation of 46 and 54 kDa SAPKs in cytosolic extracts of ventricular myocytes (Figure 1A). The order of potency of SAPK activation was studied by the in-gel kinase assay. Cells were exposed to 0�5 M sorbitol (S), 0�5 M sorbitol�0�2 lM okadaic acid (SOA), 0�2 lM okadaic acid (OA) or 50 ng/ml anisomycin (A) for 30 min at 37 �C. Okadaic acid (0�2 lM) alone caused only a marginal stimulation but enhanced the response to sorbitol.SAPK activity was immunoprecipitated from nuclear extracts of cells exposed to 0�5 M sorbitol�0�2 lM okadaic acid using a JNK1 antibody (Figure 1C)." provenance.
- _4 wasQuotedFrom 9271103 provenance.