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- _3 value "FKLF-2 also activated all the other erythroid specific promoters we tested (GATA-1, glycophorin B, ferrochelatase, porphobilinogen deaminase, and 5-aminolevulinate synthase). To test whether FKLF-2 expression changes on induction of erythroid differentiation, K562, HEL, and MEL cells were induced by hemin, delta -ALA, or hemin + HMBA,26,27 and FKLF-2 mRNA was assessed with Northern blotting. As shown in Figure 2C and 2D, induction of erythroid differentiation up-regulated FKLF-2 expression in all 3 cell lines. FKLF-2 thus differs from EKLF and FKLF, which fail to be up-regulated by inducers of erythroid differentiation.1,8" provenance.
- _3 wasQuotedFrom 10828046 provenance.