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- _4 value "We constructed a pSR-GFP-Neo expression vector by inserting an 832-bp genomic sequence that contains both miRNAs (pSR-miR-15/16-WT). The vector was transiently transfected into MEG-01 cells. The levels of Bcl2 were highly reduced (to ~7% normalized expression) in comparison with WT MEG-01 cells or cells transfected with the empty vector (which shows similar levels of Bcl2) (Fig. 2A).....As shown in Fig. 2B, separate transfections of miR-15a-sense and miR- 16-1-sense, respectively, completely abolished BCL2 expression, whereas transfection of antisense RNAs did not." provenance.
- _4 wasQuotedFrom 16166262 provenance.