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- _3 value "The data files of scanned image files hybridized with probes from RNA extracted from different time points from burned or unburned mice were converted to cell intensity (.CEL) files by using the MICROARRAY SUITE 5.0 (MAS, Affymetrix). The data were scaled to a target intensity of 500, and all possible pairwise array comparisons of the replicates to control unburned mouse were performed for each time point (e.g., four combinations when two arrays from each time point were compared to the two arrays hybridized to control RNA), using a MAS 5.0 change call algorithm. In each comparison, probe sets with a signal value difference 100 and in which one of the two samples being compared was not called Absent were scored as differentially modulated when- i the number of change calls in the same direction were at least three, four, and six when the number of comparisons were four, six, and nine, respectively; and (ii) the other comparisons were unchanged, to partially compensate for biological stochasticity and technical variation. An additional constraint of a minimum ratio of 1.65 was applied to control the known false positives at 5% [based on the ratios of 100 genes determined as invariant in most conditions tested (Affymetrix)]." provenance.
- _3 wasQuotedFrom 15809440 provenance.