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- _6 value "After activating Ras using EGF, the GTP-bound form of Ras was precipitated with GST-RBD (GST-Raf containing Ras binding domain). Precipitated Ras was detected using the HA-antibody. As shown in Fig. 5D, in the presence of EGF, the amount of GTP-bound Ras increased over that of the control. Levels of the GTP-bound Ras significantly decreased in cells expressing DIP1/2; however, DIP1/2m failed to stimulate Ras GTPase in cells treated with EGF." provenance.
- _6 wasQuotedFrom 11812785 provenance.