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- _3 value "RDA was used to study differential gene expression in macrophage-like cells (PMA-stimulated THP-1 cells) and foam-cell-like cells (PMA-stimulated and oxLDL-exposed THP-1 cells). A modified solid-phase representational difference analysis (RDA) protocol was used [7] in combination with DNA microarray analysis and reverse transcriptase PCR (RT-PCR) for evaluation. Array analysis was performed using a scanner (GMS418 Array Scanner, Genetic MicroSystems, USA) and the GenePixPro 3.0 software (Axon Instruments, USA). For data the level of significance was set to a 2-fold relative difference between samples after background subtraction. " provenance.
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