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- _3 value "Three DNA-protein complexes (C1, C3 and C4) were detected in control extracts and 4 complexes (C1, C2, C3 and C4) were detected in oncostatin M (OM)-treated nuclear extracts using a P32-labeled oligonucleotide probe (TATA1+2) containing LDLR promoter sequence -25 to +5. EMSA studies demonstrated that the C1 complex was completely supershifted by anti-c-Jun antibodies but none of these antibodies affected the C2 complex. The data suggest that the C1 complex may be a heterodimer of ATF and c-Jun; c-Jun is not present in the C2 complex. Additional gel shift assays using control extract showed a weaker supershift band with anti-c-Jun antibodies compared to the OM-treated extract, suggesting that OM stimulation may increase the c-Jun DNA-binding activity." provenance.
- _3 wasQuotedFrom 10671569 provenance.