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- _5 value "In metabolically labelled Ntva-RA cells the Akt signalling was therapeutically blocked to see the effect on synthesis of Id4. The effect of Akt blockade on the normalised polysome RNA values for Id4 frequently resulted in a shift of mRNA away from the polysome fraction and correlated well with reduction in protein synthesis. The effects on polysome recruitment occur within 2 h and at this point the effects on transcription are minimal suggesting that the transduction precedes the transcriptional effects due to the fact that 20% of the mRNA that are differentially recruited to ribosomes at 2 h encode transcription factors." provenance.
- _5 wasQuotedFrom 14580340 provenance.