Matches in Nanopublications for { <https://protocolexchange.researchsquare.com/article/pex-1069/v1#step> ?p ?o ?g. }
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- step reagentUsed CHEBI_41609 assertion.
- step reagentUsed CHEBI_18153 assertion.
- step reagentUsed CHEBI_35225 assertion.
- step reagentUsed CHEBI_29362 assertion.
- step description "Add imaging buffer with desired ratios of Buffer C (500 mM), ethylene carbonate, and IS-CF660R at 1-2 nM final concentration. The exact concentration of IS may need to be adjusted depending on the target and based on the imaging kinetics." assertion.
- step description "DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. We have developed DNA-PAINT-ERS, where E=ethylene carbonate, R=repeating sequence, and S=spacer, for fast and multiplexed superresolution imaging with DNA-PAINT. Here we describe detailed procedures for DNA-PAINT-ERS including reagent preparation, sample labeling, as well as image acqusition and analysis." assertion.
- step description "Comments can take the form of short reviews, notes or questions to the author. Comments will be posted immediately. If reported by the community, we will temporarily hide the comment to determine if the comment is spam or harassment. If it is not, it will be unhidden, and it cannot be reported again. If you think a comment has been hidden or unhidden mistakenly, please contact us at" assertion.
- step bioSampleUsed CHEBI_41609 assertion.
- step bioSampleUsed CHEBI_18153 assertion.
- step bioSampleUsed CHEBI_35225 assertion.
- step bioSampleUsed CHEBI_29362 assertion.
- step bioSampleUsed "microscopy" assertion.
- step hasExperimentalInput CHEBI_29362 assertion.
- step labEquipmentUsed CHEBI_35225 assertion.