Matches in Nanopublications for { ?s <http://www.w3.org/ns/prov#value> ?o ?g. }
- _3 value "freshly excised human skin specimens.At all stages studied the entire set of desmosomal cadherins [desmogleins (Dsg) 1-3 and desmocollins (Dsc) 1-3] was detected, with Dsg3, Dsc2 and Dsc3 being the most prominent." provenance.
- _3 value "freshly excised human skin specimens.At all stages studied the entire set of desmosomal cadherins [desmogleins (Dsg) 1-3 and desmocollins (Dsc) 1-3] was detected, with Dsg3, Dsc2 and Dsc3 being the most prominent." provenance.
- _3 value "Also, in rat aorta, nitric oxide-induced relaxation via the cGMP kinase pathway was associated with phosphorylation of PLB" provenance.
- _3 value "These in vitro studies indicate that either cAMP or cGMP can lead to phosphorylation of PLB." provenance.
- _5 value "From mutations file" provenance.
- _5 value "Modified assertion" provenance.
- _6 value "Twist expression bypassed p53-induced growth arrest. These effects correlated with an ability of Twist to interfere with activation of a p53-dependent reporter and to impair induction of p53 target genes in response to DNA damage." provenance.
- _4 value "Upon stimulation, a functional NADPH oxidase is assembled when the cytosolic proteins, Rac, p67phox, p47phox, and possibly p40phox, associate with the gp91phox and p22phox transmembrane proteins." provenance.
- _9 value "Upon stimulation, a functional NADPH oxidase is assembled when the cytosolic proteins, Rac, p67phox, p47phox, and possibly p40phox, associate with the gp91phox and p22phox transmembrane proteins." provenance.
- _6 value "In the nucleus, HDAC4 associates with the myocyte enhancer factor MEF2A. Binding of HDAC4 to MEF2A results in the repression of MEF2A transcriptional activation, a function that requires the deacetylase domain of HDAC4." provenance.
- _5 value "Our results suggest that gelsolin modulates bradykinin-mediated PLD activation via suppression of PLC and PKC activities but did not affect S1P-mediated PLD activation." provenance.
- _6 value "Wild-type but not catalytically inactive SHP-1 induced dephosphorylation of p85. Furthermore, expression of SHP-1 decreased PI3K enzyme activity in anti-phosphotyrosine immunoprecipitates and phosphorylation of serine 473 in Akt, a process dependent on PI3K activity. " provenance.
- _4 value "In the cytoplasm, it interferes with the signals to IkappaB kinases and blocks the degradation of IkappaB. In the nucleus, however, TRX enhances NF-kappaB transcriptional activities by enhancing its ability to bind DNA." provenance.
- _5 value "IFNalpha induces JAK1 auto phosphorylation." provenance.
- _7 value "Here we describe a Raf-1-interacting protein, isolated using a yeast two-hybrid screen. This protein inhibits the phosphorylation and activation of MEK by Raf-1 and is designated RKIP (Raf kinase inhibitor protein)." provenance.
- _6 value "Modified assertion" provenance.
- _3 value "Insulin stimulated AKT/PKB phosphorylation was in C2C12 myotubes." provenance.
- _5 value "Modified assertion" provenance.
- _6 value "Modified assertion" provenance.
- _6 value "Modified assertion" provenance.
- _4 value "MAb200 was injected into H460 cells along with purified activated RhoA mutant, RhoAV14 (Ridley and Hall, 1992), and cell behaviour was examined for 2 - 3 days. About 35% of injected cells underwent apoptosis in the duration of the experiment, that is a twofold reduction in the number of cell deaths compared to that obtained after injection of mAb200 alone." provenance.
- _3 value "We injected a monoclonal antibody directed against the SH3 domain of Ras-GAP (mAb200) that has been shown to block Ras-GAP downstream signalling into various human normal and tumour cell lines. When mAb200 was microinjected into lung carcinoma H460 cells, cervix carcinoma HeLa cells and colon carcinoma HCT116 cells, massive cell death occurred. Breast carcinoma SKBR3 cells, non small lung H322 and colon HT29 carcinoma cells were also sensitive to the mAb200 antibody, although to a lesser extent (data not shown)." provenance.
- _3 value "We injected a monoclonal antibody directed against the SH3 domain of Ras-GAP (mAb200) that has been shown to block Ras-GAP downstream signalling into various human normal and tumour cell lines. When mAb200 was microinjected into lung carcinoma H460 cells, cervix carcinoma HeLa cells and colon carcinoma HCT116 cells, massive cell death occurred. Breast carcinoma SKBR3 cells, non small lung H322 and colon HT29 carcinoma cells were also sensitive to the mAb200 antibody, although to a lesser extent (data not shown)." provenance.
- _8 value "These data suggest that APPL is an adaptor that may tether inactive AKT2 to p110alpha in the cytoplasm and thereby may expedite recruitment of AKT2 and p110alpha to the cell membrane upon mitogenic stimulation." provenance.
- _7 value "In addition, TGFbeta(1)- and BMP-2-induced increases in alpha1(I) collagen mRNA levels were both dependent on protein kinase C and protein tyrosine kinase activities." provenance.
- _7 value "In addition, TGFbeta(1)- and BMP-2-induced increases in alpha1(I) collagen mRNA levels were both dependent on protein kinase C and protein tyrosine kinase activities." provenance.
- _3 value "ANG II upregulates TGF-beta receptor type II expression on proximal tubular cells" provenance.
- _6 value "Treatment with the selective Src kinase inhibitor PP-2 inhibited both FAK activation and phosphorylation of FAK at Tyr(577) induced by bombesin in intact cells." provenance.
- _5 value "Treatment with the selective Src kinase inhibitor PP-2 inhibited both FAK activation and phosphorylation of FAK at Tyr(577) induced by bombesin in intact cells." provenance.
- _3 value "Androgens inhibit the growth of breast cancer cells, but the mechanism of androgen-induced growth inhibition has not yet been elucidated, and few androgen-responsive genes have been identified. We, therefore, used differential display PCR to identify novel androgen-responsive genes in ZR-75-1 human breast cancer cells. The human UDP-glucose dehydrogenase gene (UDPGDH), which was not known to be androgen regulated, was detected and cloned by complementary DNA library screening. The UDPGDH open reading frame codes for a protein of 494 amino acids that migrates at an apparent molecular mass of approximately 54 kDa. Northern blot analysis revealed the existence of two messenger RNA species of approximately 3.5 and 2.7 kb in all of the human breast cancer cell lines examined. The major UDPGDH transcript was induced rapidly (within 6 h) by dihydrotestosterone in ZR-75-1 cells, and a maximal 13-fold induction was observed after 24 h of treatment." provenance.
- _4 value "The activation of human platelets by thrombin is mediated primarily by protease-activated receptors (PARs). PAR1 and PAR4 are present on human platelets and are activated by the hexapeptides SFLLRN and GYPGQV, respectively." provenance.
- _4 value "thrombin initiates a very strong response in platelets through PAR1, leading to exposure of anionic phospholipids that support blood clotting." provenance.
- _4 value "Ability of huIL18bp to inhibit IFNgamma induction was shown by incubating human KG-1 monocytic cells with IL-18. MC53L and MC54L blocked IFNgamma production at nanogram levels in a dose dependent manner." provenance.
- _3 value "cGMP concentrations were increased significantly in penile tissue of rats transfected with AdCMVeNOS compared with control." provenance.
- _3 value "Hef1 function has been specifically implicated in signaling pathways important for cell adhesion and differentiation in lymphoid and epithelial cells" provenance.
- _4 value "On the other hand, TGF-beta stimulated the expression of both MCP-I and GM-CSF, but suppressed M-CSF expression." provenance.
- _7 value "syndecan-4 cytoplasmic domain can associate with and potentiate the activity of protein kinase C, which is required for focal adhesion formation." provenance.
- _4 value "BMP6 induced the phosphorylation of Smad5 and Smad1 in C2C12 cells and MC3T3-E1 cells." provenance.
- _5 value "It is, in particular, noteworthy that En2, which represses the BPAG1e promoter in human keratinocytes, has the opposite activating effect in fibroblasts and neuroepithelial cells. Hoxc-8 and Hoxa-5, also repressors in keratinocytes, activate BPAG1e promoter activity in COS-7 and CHP-100 cells, respectively." provenance.
- _5 value "The glypican- antisense transfected clones displayed markedly reduced glypican- protein levels and a marked attenuation of the mitogenic responses to heparin-binding growth factors that are commonly overexpressed in pancreatic cancer: fibroblast growth factor-2 (FGF2), heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF), and hepatocyte growth factor (HGF)" provenance.
- _3 value "# sentences for pubmedID=10506130: # Ariadne: Results showed that forskolin also inhibited the expression of alkaline phosphatase, bone sialoprotein, osteopontin, and osteocalcin genes (Fig. 2 C ). [Expression]" provenance.
- _3 value "# sentences for pubmedID=10506130: # Ariadne: Results showed that forskolin also inhibited the expression of alkaline phosphatase, bone sialoprotein, osteopontin, and osteocalcin genes (Fig. 2 C ). [Expression]" provenance.
- _4 value "Modified assertion" provenance.
- _5 value "Met-mediated invasive growth requires autophosphorylation of the receptor on tyrosines located in the kinase activation loop (Tyr(1234)-Tyr(1235)) and in the carboxyl-terminal tail (Tyr(1349)-Tyr(1356))" provenance.
- _3 value "Melusin is expressed in skeletal and cardiac muscles but not in smooth muscles or other tissues. Its expression is up-regulated during in vitro differentiation of the C2C12 murine myogenic cell line, and it is regulated during in vivo skeletal muscle development." provenance.
- _4 value "100 nmol/L Angiotensin II, induced EGR-1 expression was greater in newborn VSMCs (5.8-fold stimulation over basal) than in adult VSMCs (2.5-fold stimulation)." provenance.
- _5 value "Activation of PKC resulted in marked induction of NeuroD mRNA and NeuroD protein. NeuroD mRNA induction was inhibited by calphostin C, an inhibitor of PKC" provenance.
- _8 value "A large number of SH2 domain proteins have been shown to bind to PDGF alpha - and beta -receptors (Table 4). Some of these molecules are themselves enzymes, such as phosphatidylinositol 3'-kinase (PI 3-kinase), phospholipase C (PLC)-gamma , the Src family of tyrosine kinases, the tyrosine phosphatase SHP-2, and a GTPase activating protein (GAP) for Ras. Other molecules such as Grb2, Grb7, Nck, Shc, and Crk are devoid of enzymatic activity and have adaptor functions, linking the receptor with downstream catalytic molecules. Also, members of the Stat family bind to the PDGF receptors. They are transcription factors that after phosphorylation on tyrosine dimerize and translocate into the nucleus where they affect the transcription of specific genes. Each SH2 domain molecule that binds to the PDGF receptors initiates a signal transduction pathway. The more important and well-characterized signaling pathways activated by PDGF receptors are described below. For a more comprehensive discussion, see Reference 182." provenance.
- _8 value "A large number of SH2 domain proteins have been shown to bind to PDGF alpha - and beta -receptors (Table 4). Some of these molecules are themselves enzymes, such as phosphatidylinositol 3'-kinase (PI 3-kinase), phospholipase C (PLC)-gamma , the Src family of tyrosine kinases, the tyrosine phosphatase SHP-2, and a GTPase activating protein (GAP) for Ras. Other molecules such as Grb2, Grb7, Nck, Shc, and Crk are devoid of enzymatic activity and have adaptor functions, linking the receptor with downstream catalytic molecules. Also, members of the Stat family bind to the PDGF receptors. They are transcription factors that after phosphorylation on tyrosine dimerize and translocate into the nucleus where they affect the transcription of specific genes. Each SH2 domain molecule that binds to the PDGF receptors initiates a signal transduction pathway. The more important and well-characterized signaling pathways activated by PDGF receptors are described below. For a more comprehensive discussion, see Reference 182." provenance.
- _3 value "C. Atherosclerosis The involvement of PDGF in the atherosclerosis process has been confirmed experimentally using balloon catheterization of rat carotid arteries as a model. In the denuded artery, an increased amount of activated PDGF receptors is seen in the vessel wall (3, 345). The intimal thickening that follows this treatment was inhibited by administration of neutralizing PDGF antibodies (120). In addition, a low-molecular-weight PDGF receptor kinase inhibitor, AG-1295, was recently shown to inhibit neointima formation in a porcine restenosis model (25). Moreover, infusion of PDGF-BB into rats after carotid injury (210), or expression of recombinant PDGF-BB in porcine arteries (317), caused increased intimal thickening. The role of PDGF in the atherosclerotic lesions may be to stimulate smooth muscle cells to migrate from the media of the vessel to the intima layer and to proliferate and produce matrix molecules at this site (368)." provenance.
- _7 value "I. Stat The family of Stat molecules has seven members of which Stat1, Stat3, Stat5a and -beta , and Stat6 have been shown to bind to the activated PDGF beta -receptor and to be phosphorylated after PDGF stimulation; binding also occurs to the alpha -receptor, albeit only weakly (347, 474, 482). After phosphorylation on tyrosine, Stats dimerize and translocate to the nucleus, where they act as transcription factors (reviewed in Ref. 92). Stat molecules are important for the transduction of signals downstream of cytokine receptors; however, their importance in PDGF signaling remains to be elucidated." provenance.
- _7 value "I. Stat The family of Stat molecules has seven members of which Stat1, Stat3, Stat5a and -beta , and Stat6 have been shown to bind to the activated PDGF beta -receptor and to be phosphorylated after PDGF stimulation; binding also occurs to the alpha -receptor, albeit only weakly (347, 474, 482). After phosphorylation on tyrosine, Stats dimerize and translocate to the nucleus, where they act as transcription factors (reviewed in Ref. 92). Stat molecules are important for the transduction of signals downstream of cytokine receptors; however, their importance in PDGF signaling remains to be elucidated." provenance.
- _6 value "Moreover, engagement of beta 1-integrins by plating of fibroblasts on collagen or fibronectin caused a transient tyrosine phosphorylation of PDGF receptors in the absence of PDGF (459)." provenance.
- _2 value "These changes lead to the activation of five damaging events, termed perpetrators. These are the damaging actions of free radicals and their product peroxynitrite," provenance.
- _2 value "and also gene activation leading to cytokine synthesis, synthesis of enzymes involved in free radical production, and accumulation of leukocytes." provenance.
- _2 value "protein synthesis," provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _3 value "Figure 2. Differential gene expression at senesence." provenance.
- _4 value "As shown in Fig. 3, Northern blot analysis showed that the HGF levels were slightly increased in the CCl4-treated livers of the mock- and AxCALacZ-infected rats compared with those of normal rat livers. In contrast, a dramatic increase in HGF expression was observed in the rat liver infected with HNF3gamma (1.5�109 pfu)." provenance.
- _5 value "Fig. 4 shows the results of the Northern blot analysis, which indicate that endogenous HNF-3? mRNA (indicated with an arrow) was decreased 50% in the CCl4-injured liver compared with the normal rat. However, the transduced HNF-3gamma mRNA (indicated with an arrowhead) was increased dose-dependently by the AxCAHNF3gamma infection in spite of CCl4 treatment. HNF-3alpha and HNF-3beta were up-regulated in the CCl4-treated livers of mock- and AxCALacZ-infected rats. Conversely, These mRNAs in the AxCAHNF3gamma-infected liver were not observed to be induced by CCl4 treatment." provenance.
- _5 value "Modified assertion" provenance.
- _4 value "Treatment with the stem cell factor (SCF), a growth factor known to partially reduce primordial germ cell apoptosis, resulted in decreased Bax expression." provenance.
- _3 value "AGRP is a negative regulator of leptin action" provenance.
- _6 value "We conclude that glucose increases AII production by mesangial cells, which results in stimulation of TGF-beta1 secretion, decreased matrix degradation, and increased matrix accumulation. These effects of AII are mediated by the AT1 receptor. Collagenase activity in cell medium was determined using three methods: 1) zymography; 2) quantitative assay using fluoresceinated gelatin as substrate; and 3) a new enzyme-linked immunosorbent assay (ELISA) that specifically measures 72-kDa collagenase (MMP-2), the principal collagenase synthesized by mesangial cells." provenance.
- _7 value "Oncogenic Ras induces the transcription activity of NF-kappaB that confers cell survival. Oncogenic Ras also down-modulates the expression of Par-4, a transcriptional repressor protein, that is essential but not sufficient on its own to induce apoptosis. Here we show that reintroduction of Par-4 by transient transfection leads to apoptosis in cells expressing oncogenic Ras but not in those that lack oncogenic Ras expression. Par-4 abrogates oncogenic Ras-inducible NF-kappaB transcription activity" provenance.
- _5 value "Oncogenic Ras induces the transcription activity of NF-kappaB that confers cell survival. Oncogenic Ras also down-modulates the expression of Par-4, a transcriptional repressor protein, that is essential but not sufficient on its own to induce apoptosis. Here we show that reintroduction of Par-4 by transient transfection leads to apoptosis in cells expressing oncogenic Ras but not in those that lack oncogenic Ras expression. Par-4 abrogates oncogenic Ras-inducible NF-kappaB transcription activity" provenance.
- _5 value "H2320 was transfected (together with pRlSV40) into MCT cells, together with empty expression vector (vector), expression vectors for WT c-Ets2 a mutant c-Ets2 that cannot be phosphorylated by ERK1/2" provenance.
- _4 value "Interestingly, treatment with specific p38 mitogen-activated protein kinase inhibitors blocks both myotube formation and caveolin-3 expression" provenance.
- _5 value "It was previously shown that ephrin-A2, -A3 and -A5 bind to, and activate the EphA8 receptor tyrosine kinase, respectively." provenance.
- _4 value "Modified assertion" provenance.
- _5 value "By contrast, G-protein inhibition by pertussis toxin, or transfection with a dominant negative mutant of Ha-Ras strongly increased fibronectin-induced MMP-2 and MMP-9. Inhibition of PI3 kinase, MAPkinase (MEK1), or p38 MAPkinase by wortmannin, PD 98059, or SB 202190, respectively, strongly promoted fibronectin-induced MMP2 and MMP-9" provenance.
- _5 value "Transient transfection study revealed that p120 enhanced the transcriptional activation of PPARgamma/RXR induced by PPARgamma- or RXR-specific ligands. The yeast two-hybrid analysis showed no interaction of p120 with PPARgamma under any conditions, and electophoretic mobility shift assay showed apparent DNA-PPARgamma/RXR/p120 complex formation only in the presence of 9-cis-RA." provenance.
- _5 value "3-phosphoinositide-dependent protein kinase-1 (PDK-1) is required for activation of atypical PKCs. Overexpression of wild-type PDK-1 provoked increases in the activity of cotransfected hemagglutinin (HA)-tagged PKC-zeta and concomitantly enhanced HA-tagged GLUT4 translocation... Thr-410, the immediate target of PDK-1 in the activation loop of PKC-zeta" provenance.
- _3 value "SLUG is an evolutionarily conserved transcriptional repressor whose activation by E2A-HLF promotes the aberrant survival and eventual malignant transformation of mammalian pro-B cells otherwise slated for apoptotic death." provenance.