Matches in Nanopublications for { ?s <http://www.w3.org/ns/prov#value> ?o ?g. }
- _3 value "The Sir2 histone deacetylase gene family consists of seven mammalian sirtuins (SIRTs) which are NAD-dependent histone/protein deacetylases. Sir2 proteins regulate, for instance, genome stability by chromatin silencing in yeast. In mammals, their function is still largely unknown. Due to the NAD+ dependency, Sir2 might be the link between metabolic activity and histone/protein acetylation. Regulation of gene expression also seems to play an important role in Sir2 functions, since increasing the dosage of Sir2 genes increases genome stability in yeast and Caenorhabditis elegans. We observed that the modification of histone/protein acetylation status by several class I and II histone deacetylase (HDAC) inhibitors induces differential changes in gene expression profiles of seven SIRT mRNAs in cultured neuronal cells. SIRT2, SIRT4 and SIRT7 were upregulated, whereas SIRT1, SIRT5 and SIRT6 were downregulated by trichostatin A (TSA) and n-butyrate. The upregulation of SIRT mRNAs was inhibited by actinomycin D. Interestingly, the regulation of SIRT mRNAs was highly similar both in mouse Neuro-2a neuroblastoma cells and post-mitotic rat primary hippocampal and cerebellar granule neurons. Using a chromatin immunoprecipitation technique, we showed that the upregulation of SIRT2 expression with TSA is related to the hyperacetylation of DNA-bound histone H4 within the first 500 bp upstream of the transcription start site of the SIRT2 gene. Chemically different types of HDAC inhibitors, such as TSA, apicidin, SAHA, M344 and n-butyrate induced remarkably similar responses in SIRT1-7 mRNA expression patterns. Differential responses in SIRT mRNA expression profiles indicate that the expression of the Sir2 family of genes is selectively regulated and dependent on histone/protein acetylation status." provenance.
- _3 value "The Sir2 histone deacetylase gene family consists of seven mammalian sirtuins (SIRTs) which are NAD-dependent histone/protein deacetylases. Sir2 proteins regulate, for instance, genome stability by chromatin silencing in yeast. In mammals, their function is still largely unknown. Due to the NAD+ dependency, Sir2 might be the link between metabolic activity and histone/protein acetylation. Regulation of gene expression also seems to play an important role in Sir2 functions, since increasing the dosage of Sir2 genes increases genome stability in yeast and Caenorhabditis elegans. We observed that the modification of histone/protein acetylation status by several class I and II histone deacetylase (HDAC) inhibitors induces differential changes in gene expression profiles of seven SIRT mRNAs in cultured neuronal cells. SIRT2, SIRT4 and SIRT7 were upregulated, whereas SIRT1, SIRT5 and SIRT6 were downregulated by trichostatin A (TSA) and n-butyrate. The upregulation of SIRT mRNAs was inhibited by actinomycin D. Interestingly, the regulation of SIRT mRNAs was highly similar both in mouse Neuro-2a neuroblastoma cells and post-mitotic rat primary hippocampal and cerebellar granule neurons. Using a chromatin immunoprecipitation technique, we showed that the upregulation of SIRT2 expression with TSA is related to the hyperacetylation of DNA-bound histone H4 within the first 500 bp upstream of the transcription start site of the SIRT2 gene. Chemically different types of HDAC inhibitors, such as TSA, apicidin, SAHA, M344 and n-butyrate induced remarkably similar responses in SIRT1-7 mRNA expression patterns. Differential responses in SIRT mRNA expression profiles indicate that the expression of the Sir2 family of genes is selectively regulated and dependent on histone/protein acetylation status." provenance.
- _4 value "Herein, DACH1 was found to be expressed in breast cancer cell lines and to inhibit transforming growth factor-beta (TGF-beta)-induced apoptosis. DACH1 repressed TGF-beta induction of AP-1 and Smad signaling in gene reporter assays and repressed endogenous TGF-beta-responsive genes by microarray analyses" provenance.
- _3 value "Table 2. Gene expression in WS and normal old cells relative to normal young cells" provenance.
- _3 value "Table 2. Gene expression in WS and normal old cells relative to normal young cells" provenance.
- _3 value "Table 2. Gene expression in WS and normal old cells relative to normal young cells" provenance.
- _3 value "Table 2. Gene expression in WS and normal old cells relative to normal young cells" provenance.
- _3 value "Table 2. Gene expression in WS and normal old cells relative to normal young cells" provenance.
- _3 value "Table 2. Gene expression in WS and normal old cells relative to normal young cells" provenance.
- _3 value "Table 2. Gene expression in WS and normal old cells relative to normal young cells" provenance.
- _3 value "Table 2. Gene expression in WS and normal old cells relative to normal young cells" provenance.
- _3 value "Table 2. Gene expression in WS and normal old cells relative to normal young cells" provenance.
- _3 value "Table 2. Gene expression in WS and normal old cells relative to normal young cells" provenance.
- _3 value "Table 2. Gene expression in WS and normal old cells relative to normal young cells" provenance.
- _3 value "Table 2. Gene expression in WS and normal old cells relative to normal young cells" provenance.
- _3 value "Table 2. Gene expression in WS and normal old cells relative to normal young cells" provenance.
- _5 value "Modified assertion" provenance.
- _4 value "FIGURE 2. Activation of TLR2 and TLR4 by Y. enterocolitica infection." provenance.
- _4 value "inflammatory fluids. Chemerin is secreted as a precursor of low biological activity, which upon proteolytic cleavage of its COOH-terminal domain, is converted into a potent and highly specific agonist of ChemR23, the chemerin receptor. Activation of chemerin receptor results in intracellular calcium release, inhibition of cAMP accumulation, and phosphorylation of p42-p44 MAP kinases, through the Gi class of heterotrimeric G proteins." provenance.
- _5 value "The activation domain of Pax2 is phosphorylated by the c-Jun N-terminal kinase (JNK) to enhance Pax2-dependent transcription." provenance.
- _3 value "Table 2. Most markedly up- and downregulated genes during the time course of decidualization in response to 8-Br-cAMP Ontologic Category/ Gene Name 2 h 12 h 24 h 36 h 48 h A: UPREGULATED GENES Neuropeptides Preprosomatostatin 115.49 116.39 1644.4 1396.82 1324.2 L-orphan (clone 5) G- 3.62 6.56 79.77 126.18 123.26 protein coupled R Immune genes IL-11 7.1 2.62 28.3 74.9 62.98 Factor XIII precursor NA NA 26.98 28.78 25.815 Cell growth/ differentiation Prolactin NA 3.88 99.45 143.41 37.135 IGFBP-1 8.38 8.375 142.52 140.54 115.78 EBAF NA 3.48 70.49 123.86 51.37 NGFI-B/nur77 transcr. factor 106.4 NA 17.23 14.11 30.12 NOT 350.94 2.24 24.73 30.19 113.14 Extracellular matrix protease MMP-10 (stromelysin-2) 4.25 2.13 148.48 144.2 69.71 B: DOWNREGULATED GENES Cell growth IGFBP-5 NA 8.925 8.81 9.015 80.185 Enzymes L-Kynurenine hydrolase 7.61 NA 3.33 6.9 169.32 Signal transduction Activates NF-{kappa} B 7.87 17.51 2.19 4.18 369.01 Structural protein Actin, tropomyosin, calmodulin binding protein NA 22.32 13.17 12.59 50.21 Cell cycle Cyclin B2 NA 5.69 9.07 5.17 10.2 Cyclin E2 2.12 NA NA NA 9.28" provenance.
- _3 value "Table 2. Most markedly up- and downregulated genes during the time course of decidualization in response to 8-Br-cAMP Ontologic Category/ Gene Name 2 h 12 h 24 h 36 h 48 h A: UPREGULATED GENES Neuropeptides Preprosomatostatin 115.49 116.39 1644.4 1396.82 1324.2 L-orphan (clone 5) G- 3.62 6.56 79.77 126.18 123.26 protein coupled R Immune genes IL-11 7.1 2.62 28.3 74.9 62.98 Factor XIII precursor NA NA 26.98 28.78 25.815 Cell growth/ differentiation Prolactin NA 3.88 99.45 143.41 37.135 IGFBP-1 8.38 8.375 142.52 140.54 115.78 EBAF NA 3.48 70.49 123.86 51.37 NGFI-B/nur77 transcr. factor 106.4 NA 17.23 14.11 30.12 NOT 350.94 2.24 24.73 30.19 113.14 Extracellular matrix protease MMP-10 (stromelysin-2) 4.25 2.13 148.48 144.2 69.71 B: DOWNREGULATED GENES Cell growth IGFBP-5 NA 8.925 8.81 9.015 80.185 Enzymes L-Kynurenine hydrolase 7.61 NA 3.33 6.9 169.32 Signal transduction Activates NF-{kappa} B 7.87 17.51 2.19 4.18 369.01 Structural protein Actin, tropomyosin, calmodulin binding protein NA 22.32 13.17 12.59 50.21 Cell cycle Cyclin B2 NA 5.69 9.07 5.17 10.2 Cyclin E2 2.12 NA NA NA 9.28" provenance.
- _5 value "Modified assertion" provenance.
- _4 value "Glucose (up to 35mM) increased secretion of PAI-1 (p<0.01) and IL-8 (p<0.01), but not TNF-alpha, in a dose- and time-dependent manner." provenance.
- _3 value "Glucose (up to 35mM) increased secretion of PAI-1 (p<0.01) and IL-8 (p<0.01), but not TNF-alpha, in a dose- and time-dependent manner." provenance.
- _6 value "We demonstrate that BRCA1 and FHL2 can physically associate in vitro, BRCA1 enhanced FHL2-mediated transcriptional activity in transient transfections. Tumor-derived transactivation-deficient BRCA1 mutants showed a reduced ability to enhance transactivation by FHL2. Lack of BRCA1 binding sites in the FHL2 completely abolished the FHL2 transactivation function." provenance.
- _7 value "Treatment of keratinocytes with epidermal growth factor results in an increase in Delta Np63 alpha expression at the mRNA level, which is abrogated by inhibition of PI3K but not mitogen-activated protein kinase signaling." provenance.
- _3 value "Taken together, these results suggest that IRS1 inhibitory serine phosphorylation is a key component of insulin resistance and its reversal contributes to the insulin sensitizing effects by rosiglitazone." provenance.
- _4 value "Activation of p44/42 appeared to be antiapoptotic, since MAPK stimulation with epidermal growth factor or a dominant-positive p42 construct inhibited apoptosis." provenance.
- _6 value "The impact of the Y500F mutation in IL4Ralpha on Th cell differentiation was examined by comparing the in vitro differentiation of wildtype and Y500F T cells into Th1 and Th2 effector lymphocytes in the presence of IL12 and IL4 respectively. The residual production Th2 cytokine IL13 was significantly decreased in Y500F T cell cultures that have been differentiated under Th1 polarizing conditions compared with their wildtype counterparts. This suggests that the Y500 pathway modulated Th cell differentiation by promoting the persistence of Th2 cells in the context of Th1 skewing conditions." provenance.
- _3 value "Estradiol increased PTEN phosphorylation at 5-15 min. After 24-h treatment, progesterone induced a significant increase in PTEN protein levels, assessed by Western blot." provenance.
- _3 value "In the septal region, we found an unchanged mRNA expression after corticosterone treatment, whereas in the hippocampus there was a general increase in mRNA. Particularly, the gene expression of NGF, NT-3, and the high affinity receptors trkA, trkB and trkC increased significantly" provenance.
- _3 value "In the septal region, we found an unchanged mRNA expression after corticosterone treatment, whereas in the hippocampus there was a general increase in mRNA. Particularly, the gene expression of NGF, NT-3, and the high affinity receptors trkA, trkB and trkC increased significantly" provenance.
- _4 value "The phosphorylated form of Hsp27 was increased in response to 20micro g/ml thrombospondin 1." provenance.
- _4 value "Here we show that the type II arginine-specific methyltransferase PRMT5, which is involved in cyclin E repression, can be found in association with Brg1 and hBrm-based hSWI/SNF complexes." provenance.
- _5 value "the mechanism by which fibroblast growth factor 2 (FGF-2) protects small cell lung cancer (SCLC) cells from etoposide-induced cell death involves inhibition of Smac release but not of cytochrome c release. This process is MEK dependent" provenance.
- _6 value "Here we show that inhibition of this kinase involves atypical PKCzeta, which physically interacts with PKB in unstimulated cells. Insulin reduces the PKB-PKCzeta interaction and stimulates PKB. However, dissociation of the kinase complex and the attendant hormonal activation of PKB were prevented by ceramide." provenance.
- _4 value "Modified assertion" provenance.
- _4 value "ATF4 is required for the normal differentiation of the lamina propria layer of the vas deferens" provenance.
- _5 value "Treatment of IKK2 deleted macrophages with 10 ng/ml lipopolysaccharide (LPS, an activator of IKBKB activity) for 3 hrs showed a strong reduction in total IL-6 production as these cells are completely lacking NF-KB activation due to absence of IKK2. IL-6 production was reduced in IKK2 del macrophages at 3 and 6 hours, respectively after stimulation, but this difference disappears after 24 hours." provenance.
- _6 value "Treatment of IKK2 mutant macrophages with lippolysaccharides (LPS, an activator of IKBKB activity) showed approximately 40-50% less p65 activation at 10 and 60 minutes, respectively when compared with control. In addition, immunocytochemistry revealed that approximately 20% of IKK2 deleted cells did not show nuclear translocation of p65 after LPS stimulation, whereas control showed >95% translocation. This indicates that LPS induced activation of p65 was mediated by IKK2." provenance.
- _3 value "Figure 7 Model of PMN NADPH oxidaseâ�?��??derived oxidant signaling in mediating the TLR4-TLR2 cross talk in endothelial cells. LPS stimulation induces NADPH oxidase activation and production of reactive oxygen species (ROS) in PMNs as well as the initiation of MyD88-dependent NF-�?ºB signaling in endothelial cells and the consequent expression of TLR2 and ICAM-1. Adhesion of PMNs to endothelial cells is mediated by binding of constitutive ICAM-1 to CD18 integrin and provides the appropriate coupling required for PMNs to transmit oxidant signals to endothelial cells. The oxidants augment NF-�?ºB signaling and TLR2 expression (+), which results in the augmented response of the cell to PGN, thereby amplifying ICAM-1 expression (circled +) and promoting stable adhesion of PMNs to endothelial cells and increased PMN migration. Thus, the PMN NADPH oxidaseâ�?��??mediated TLR4-TLR2 cross talk activates a positive feedback signal leading to sustained and amplified endothelial activation in response to invading pathogens." provenance.
- _6 value "Superoxide dismutase and glutathione peroxidase prevented H/R-induced IL-1 and IL-6 increase" provenance.
- _3 value "a dominant-negative form of RUNX1 protects U937 cells from apoptotic stimuli previously shown to be dependent on protein kinase Cbeta." provenance.
- _5 value "TABLE II Genes regulated by HOXA9" provenance.
- _5 value "TABLE II Genes regulated by HOXA9" provenance.
- _5 value "TABLE II Genes regulated by HOXA9" provenance.
- _4 value "The overexpression of ATF4 also provoked accumulation of beta-catenin, but to a lower level than that resulting from the expression of Vpu." provenance.
- _4 value "2 structurally distinct factions of cellular relatives instead elicit cell death one family contains Bax, Bak and Bok, which share 3 domains" provenance.
- _5 value "Cells from mice deficient in caspase-12 were partially resistant to ER stress-induced apoptosis but still succumbed to other death stimuli." provenance.
- _5 value "Modified assertion" provenance.
- _4 value "Inhibition of Nek6 function by either overexpression of an inactive Nek6 mutant or elimination of endogenous Nek6 by siRNA arrests cells in M phase and triggers apoptosis." provenance.
- _4 value "The density of beta(2)-adrenoceptors increased in both the atria and the ventricles" provenance.
- _3 value "Estradiol induced a significant reduction in uterine TIMP-3 expression in wild-type mice" provenance.
- _3 value "It may also provide another example of the multifaceted anti-inflammatory effects of C1INH in various animal models and human diseases." provenance.
- _5 value "Insulin increased PLCgamma1 tyrosine phosphorylation at Tyr-783 and its colocalization with the IR in punctated structures enriched in cortical actin at the dorsal plasma membrane." provenance.
- _5 value "Figure 6 Notch function in cervical cancer. A theoretical model of how the tumour-suppressor properties of NOTCH1 can, in principle, be sidestepped in HPV-infected cervical cancer cells, allowing manifestation of growth-promoting properties of NOTCH1. Apart from HPV E6 and E7 proteins, cervical cancers frequently express increased levels of the phosphatidylinositol 3-kinase (PI3K) subunit p110? and ?Np63 as a result of amplification of the q26-29 locus of chromosome 3. Experiments show that Notch-induced PI3K confers resistance to anoikis in epithelial cells. Whether ERBB2 is induced by NOTCH in cervical cancer remains to be elucidated." provenance.
- _6 value "Figure 6 Notch function in cervical cancer. A theoretical model of how the tumour-suppressor properties of NOTCH1 can, in principle, be sidestepped in HPV-infected cervical cancer cells, allowing manifestation of growth-promoting properties of NOTCH1. Apart from HPV E6 and E7 proteins, cervical cancers frequently express increased levels of the phosphatidylinositol 3-kinase (PI3K) subunit p110? and ?Np63 as a result of amplification of the q26-29 locus of chromosome 3. Experiments show that Notch-induced PI3K confers resistance to anoikis in epithelial cells. Whether ERBB2 is induced by NOTCH in cervical cancer remains to be elucidated." provenance.
- _6 value "Notch1 exerts its tumour-suppressive effect in the mouse skin partially by repressing components of the Wnt (see a) and Shh (see b) signalling pathways.Activation of both pathways has been associated with many forms of cancer.Activation of WNT signalling has been linked with colorectal cancer, hepatocellular tumours, pilomatricomas, melanomas and other types of cancer (for a review, see REF. 98).Aberrant SHH signalling has been associated with sporadic basal-cell carcinoma, medulloblastoma and rhabdomyosarcoma (for a review, see REF. 138)." provenance.
- _8 value "The diagram depicts the Sonic hedgehog (SHH)-GLI pathway and shows the transduction of the SHH signal through the Smoothend (SMO)/Patched (PTC) receptor complex. In the absence of the SHH ligand,PTC represses SMO. Signalling is initiated by the binding of SHH to PTC,which relieves the repression of SMO. Subsequently, SMO signals to the nucleus by increasing the concentration of GLI transcription factors,which recruit co-activators to activate downstream target genes." provenance.
- _10 value "The diagram depicts the Sonic hedgehog (SHH)-GLI pathway and shows the transduction of the SHH signal through the Smoothend (SMO)/Patched (PTC) receptor complex. In the absence of the SHH ligand,PTC represses SMO. Signalling is initiated by the binding of SHH to PTC,which relieves the repression of SMO. Subsequently, SMO signals to the nucleus by increasing the concentration of GLI transcription factors,which recruit co-activators to activate downstream target genes." provenance.
- _6 value "The diagram depicts the Sonic hedgehog (SHH)-GLI pathway and shows the transduction of the SHH signal through the Smoothend (SMO)/Patched (PTC) receptor complex. In the absence of the SHH ligand,PTC represses SMO. Signalling is initiated by the binding of SHH to PTC,which relieves the repression of SMO. Subsequently, SMO signals to the nucleus by increasing the concentration of GLI transcription factors,which recruit co-activators to activate downstream target genes." provenance.
- _3 value "As a new line of inquiry into the molecular mechanisms underlying pathophysiological processes associated with angiotensin (ANG II)-dependent hypertension, we applied the method of serial analysis of gene expression (SAGE) to examine genome-wide transcription changes in the kidneys of mice that developed hypertension in response to chronic ANG II administration. Mice were infused subcutaneously via osmotic minipumps with ANG II for 7 days, and systolic blood pressure was measured by tail-cuff plethysmography. Subsequently, mice were euthanized, and the total RNA isolated from the kidneys was used to construct SAGE libraries. Comparison of 11,447 SAGE tags from the hypertensive kidneys, representing 5,740 unique transcripts, and 11,273 tags from the control kidneys, corresponding to 5,619 different transcripts, identified genes that are significantly (P upregulated in the hypertensive kidney. Our assessment of the genome-wide influence of ANG II resulted in the detection of several novel genes and in a recognition of potential new roles for the previously characterized genes, thus providing new probes with which to further explore the ANG II effects in normal and disease states." provenance.
- _3 value "As a new line of inquiry into the molecular mechanisms underlying pathophysiological processes associated with angiotensin (ANG II)-dependent hypertension, we applied the method of serial analysis of gene expression (SAGE) to examine genome-wide transcription changes in the kidneys of mice that developed hypertension in response to chronic ANG II administration. Mice were infused subcutaneously via osmotic minipumps with ANG II for 7 days, and systolic blood pressure was measured by tail-cuff plethysmography. Subsequently, mice were euthanized, and the total RNA isolated from the kidneys was used to construct SAGE libraries. Comparison of 11,447 SAGE tags from the hypertensive kidneys, representing 5,740 unique transcripts, and 11,273 tags from the control kidneys, corresponding to 5,619 different transcripts, identified genes that are significantly (P upregulated in the hypertensive kidney. Our assessment of the genome-wide influence of ANG II resulted in the detection of several novel genes and in a recognition of potential new roles for the previously characterized genes, thus providing new probes with which to further explore the ANG II effects in normal and disease states." provenance.
- _3 value "KLF5 mediates a novel distinct delayed persistent induction of PDGF-A chain in response to the model agonist, phorbol ester, through a cis-element previously shown to mediate phorbol ester induction on to PDGF-A chain through the early growth response factor (Egr-1)" provenance.
- _3 value "Table 1. List of Genes Up-Regulated in NB4 Cells by ATRA" provenance.
- _3 value "Table 1. List of Genes Up-Regulated in NB4 Cells by ATRA" provenance.
- _3 value "Table 1. List of Genes Up-Regulated in NB4 Cells by ATRA" provenance.
- _3 value "Table 2. List of Genes Down-Regulated in NB4 Cells by ATRA" provenance.
- _3 value "Table 2. List of Genes Down-Regulated in NB4 Cells by ATRA" provenance.
- _3 value "Table 2. List of Genes Down-Regulated in NB4 Cells by ATRA" provenance.
- _3 value "Table 2. List of Genes Down-Regulated in NB4 Cells by ATRA" provenance.
- _3 value "Histamine treatment significantly increased the expression of H2R mRNA in iDC and H1R mRNA in LPS-activated DC" provenance.
- _3 value "In the presence of tumor necrosis factor (TNF)-alpha histamine also increased IL-12 p40 and IL-6 production." provenance.
- _3 value "When expressions of histamine H1 (H1R) and H2 (H2R) receptors in DC were analyzed by RT-PCR, both receptors were down-regulated after LPS or TNF-alpha" provenance.
- _5 value "histamine treatment decreased the expression of both histamine receptors in TNF-alpha-stimulated DC" provenance.
- _4 value "In this study, we show that PAK1 can be stimulated by carbachol, lysophosphatidic acid (LPA), epidermal growth factor (EGF), and phorbol 12-myristate 13-acetate (PMA) by multiple independent and overlapping pathways." provenance.
- _5 value "Studies using inhibitors of the EGF receptor tyrosine kinase, phosphatidylinositol 3-kinase (PI3-kinase) and protein kinase C (PKC) revealed that all of the cell surface agonists could activate PAK1 through pathways independent of PKC, that EGF stimulated a PI3-kinase dependent pathway to stimulate PAK1, and that muscarinic receptor stimulation of PAK1 was predominantly mediated through this EGF-R-dependent mechanism" provenance.
- _4 value "The androgen receptor (AR), when complexed with 5alpha-dihydrotestosterone (DHT), supports the survival and proliferation of prostate cells, a process critical for normal development, benign prostatic hypertrophy, and tumorigenesis." provenance.
- _5 value "Neurogenin3 bound to and effectively activated transcription through the nkx2.2 and PAX4 E boxes. In contrast, Neurogenin3 strongly repressed the NEUROG3 promoter, although a proximal E box was required for activity in the absence of Neurogenin3, suggesting that a ubiquitous transcriptional activator may bind to this site, and that Neurogenin3 could act as a competitive inhibitor of this activator. " provenance.
- _3 value "Taxol increased the expression of E-cadherin and nm23." provenance.
- _5 value "we show that CDT1, a licensing factor of the pre-replication complex (preRC), is rapidly proteolysed after UV- or gamma-irradiation." provenance.
- _4 value "We now show that UV-induced NF-kappaB activation depends on phosphorylation of IkappaBalpha at a cluster of C-terminal sites that are recognized by CK2 (formerly casein kinase II)." provenance.
- _5 value "In metabolically labelled Ntva-RA cells the Akt signalling was therapeutically blocked to see the effect on synthesis of Igfbp5. The effect of Akt blockade on the normalised polysome RNA values for Igfbp5 frequently resulted in a shift of mRNA away from the polysome fraction and correlated well with reduction in protein synthesis. The effects on polysome recruitment occur within 2 h and at this point the effects on transcription are minimal suggesting that the transduction precedes the transcriptional effects due to the fact that 20% of the mRNA that are differentially recruited to ribosomes at 2 h encode transcription factors." provenance.
- _5 value "In metabolically labelled Ntva-RA cells the Akt signalling was therapeutically blocked to see the effect on synthesis of Notch1. The effect of Akt blockade on the normalised polysome RNA values for Notch1 frequently resulted in a shift of mRNA away from the polysome fraction and correlated well with reduction in protein synthesis. The effects on polysome recruitment occur within 2 h and at this point the effects on transcription are minimal suggesting that the transduction precedes the transcriptional effects due to the fact that 20% of the mRNA that are differentially recruited to ribosomes at 2 h encode transcription factors." provenance.
- _5 value "cell growth" provenance.
- _5 value "miscellaneous" provenance.
- _5 value "miscellaneous" provenance.
- _5 value "miscellaneous" provenance.
- _5 value "proteolysis" provenance.
- _5 value "proteolysis" provenance.
- _5 value "serum proteins" provenance.
- _5 value "we performed microarray analyses of differential gene expression in the liver between wild type and Stra13-/- mice and identified 42 target genes including a subset of 20 previously known as clock-controlled genes metabolism genes" provenance.
- _5 value "we performed microarray analyses of differential gene expression in the liver between wild type and Stra13-/- mice and identified 42 target genes including a subset of 20 previously known as clock-controlled genes metabolism genes" provenance.
- _5 value "we performed microarray analyses of differential gene expression in the liver between wild type and Stra13-/- mice and identified 42 target genes including a subset of 20 previously known as clock-controlled genes metabolism genes" provenance.
- _6 value "heterologously expressed {alpha} and beta4 BK channels could be activated by 17beta-estradiol BKalpha/beta4 channels were not blocked by 100 nM charybdotoxin or iberiotoxin, and were activated by 17beta-estradiol." provenance.
- _3 value "In vitro experiments using purified recombinant ARP4 protein revealed that ARP4 markedly inhibited the proliferation, chemotaxis, and tubule formation of endothelial cells." provenance.
- _3 value "Angiogenic defects in Id mutant mice" provenance.
- _5 value "Ligation of CD47 by its natural ligand thrombospondin (TSP), or cross-linking by CD47 antibodies, triggers caspase-independent cell death in normal and leukemic cells." provenance.
- _5 value "The NF-kappaB pathway is important in the control of the immune and inflammatory response. One of the critical events in the activation of this pathway is the stimulation of the IkappaB kinases (IKKs) by cytokines such as tumor necrosis factor-alpha and interleukin-1." provenance.